1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Cadherins
  5. Cadherin-16
  6. CDH16 Protein, Human (HEK293, His)

CDH16 Protein, Human (HEK293, His)

Cat. No.: HY-P73507
COA Handling Instructions

CDH16, a calcium-dependent cell adhesion protein, belongs to the cadherin family. It exhibits homophilic interactions, potentially contributing to cell-type sorting and regulating cell-cell adhesions for cellular organization. CDH16 Protein, Human (HEK293, His) is the recombinant human-derived CDH16 protein, expressed by HEK293 , with C-His labeled tag. The total length of CDH16 Protein, Human (HEK293, His) is 768 a.a., with molecular weight of 90-110 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

CDH16, a calcium-dependent cell adhesion protein, belongs to the cadherin family. It exhibits homophilic interactions, potentially contributing to cell-type sorting and regulating cell-cell adhesions for cellular organization. CDH16 Protein, Human (HEK293, His) is the recombinant human-derived CDH16 protein, expressed by HEK293 , with C-His labeled tag. The total length of CDH16 Protein, Human (HEK293, His) is 768 a.a., with molecular weight of 90-110 kDa.

Background

CDH16, a member of the cadherin family, is a calcium-dependent cell adhesion protein. It exhibits a preference for homophilic interactions, engaging with itself in connecting cells. This characteristic suggests that CDH16, like other cadherins, may play a role in the sorting of heterogeneous cell types, contributing to the regulation of cell-cell adhesions and maintaining cellular organization.

Biological Activity

Immobilized Human CDH16, His Tag at 0.5 μg/mL (100μl/well) on the plate. Dose response curve for Anti-CDH16 Antibody, Mouse IgG Tag with the EC50 of ≤7.5 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-His

Accession

O75309/NP_004053.1 (K19-A786)

Gene ID
Molecular Construction
N-term
CDH16 (K19-A786)
Accession # O75309/NP_004053.1
His
C-term
Synonyms
Cadherin-16; Ksp-cadherin; CDH16
Molecular Weight

90-110 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CDH16 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CDH16 Protein, Human (HEK293, His)
Cat. No.:
HY-P73507
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