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  4. Transferases (EC 2)
  5. CHAC1 Protein, Human

CHAC1 Protein, Human

Cat. No.: HY-P701945
Handling Instructions

The CHAC1 protein cleaves glutathione, inducing apoptosis via the ATF4-ATF3-DDIT3/CHOP cascade. It acts as a negative regulator of the Notch signaling pathway, promoting neurogenesis by inhibiting Notch cleavage. CHAC1 Protein, Human is the recombinant human-derived CHAC1 protein, expressed by E. coli , with tag free. The total length of CHAC1 Protein, Human is 221 a.a., .

For research use only. We do not sell to patients.

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Description

The CHAC1 protein cleaves glutathione, inducing apoptosis via the ATF4-ATF3-DDIT3/CHOP cascade. It acts as a negative regulator of the Notch signaling pathway, promoting neurogenesis by inhibiting Notch cleavage. CHAC1 Protein, Human is the recombinant human-derived CHAC1 protein, expressed by E. coli , with tag free. The total length of CHAC1 Protein, Human is 221 a.a., .
Background

CHAC1, a key player in cellular processes, catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide, exhibiting specificity for glutathione over other gamma-glutamyl peptides. Its enzymatic action on glutathione is crucial as glutathione depletion is a significant factor in apoptosis initiation and execution. CHAC1 further serves as a pro-apoptotic component within the unfolded protein response pathway, mediating the effects of the ATF4-ATF3-DDIT3/CHOP cascade. Additionally, CHAC1 functions as a negative regulator of the Notch signaling pathway, impacting embryonic neurogenesis by inhibiting Notch cleavage through furin. This inhibition maintains Notch in an immature, inactive form, thereby promoting neurogenesis in embryos.
Species

Human
Source

E. coli
Tag

Tag Free
Accession

Q9BUX1 (K2-V222)
Gene ID

79094
Molecular Construction
N-term
CHAC1 (K2-V222)
Accession # Q9BUX1
C-term
Synonyms
CHAC1; Glutathione-specific gamma-glutamylcyclotransferase 1; Gamma-GCG 1; Blocks Notch protein; Botch; Cation transport regulator-like protein 1
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CHAC1 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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