DHODH Protein, Rat (Cell-Free, His)

DHODH is a key enzyme that uses quinone as an electron acceptor to catalyze the conversion of dihydroorotate into orotic acid, playing a key role in cell metabolism. This enzyme activity is indispensable for the biosynthesis of UMP (uridine monophosphate) via the de novo pathway, contributing to the fundamental process of nucleotide production and maintenance of balance in the nucleotide pool. DHODH Protein, Rat (Cell-Free, His) is the recombinant rat-derived DHODH protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of DHODH Protein, Rat (Cell-Free, His) is 395 a.a., with molecular weight of 44.2 kDa.

Dihydroorotate dehydrogenase (DHODH) is a key enzyme crucial for the de novo biosynthesis of UMP (uridine monophosphate). Functioning at a pivotal step in this pathway, DHODH catalyzes the conversion of dihydroorotate to orotate, employing quinone as an electron acceptor. This enzymatic activity is essential for the synthesis of UMP, a fundamental building block for RNA and DNA. By participating in the de novo pathway of pyrimidine biosynthesis, DHODH plays a critical role in providing the necessary nucleotide precursors for vital cellular processes, including DNA replication and RNA synthesis. The reliance on quinone as an electron acceptor underscores the intricate redox chemistry involved in DHODH function, highlighting its significance in maintaining the cellular pool of pyrimidine nucleotides and supporting essential cellular functions.

Rat

E. coli Cell-free

N-10*His

Q63707 (M1-R395)

65156

MAWRQLRKRALDAVIILGGGGLLFTSYLTATGDDHFYAEYLMPGLQRLLDPESAHRLAVRVTSLGLLPRATFQDSDMLEVKVLGHKFRNPVGIAAGFDKNGEAVDGLYKLGFGFVEVGSVTPQPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAQLTADGLPLGINLGKNKTSEDAAADYAEGVRTLGPLADYLVVNVSSPNTAGLRSLQGKTELRHLLSKVLQERDALKGTRKPAVLVKIAPDLTAQDKEDIASVARELGIDGLIVTNTTVSRPVGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRIPIIGVGGVSSGQDALEKIQAGASLVQLYTALIFLGPPVVVRVKRELEALLKERGFTTVTDAIGADHRR

44.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.