EphA4 Protein, Human (HEK293)

The EphA4 protein is a receptor tyrosine kinase that participates in bidirectional signaling with membrane-bound ephrin ligands including EFNA1 and EFNB3. EphA4 is activated by GPI-anchored ephrin A and transmembrane ephrin B and regulates cell morphology and integrin-dependent adhesion through regulation of Rac, Rap, and Rho GTPases. EphA4 Protein, Human (HEK293) is the recombinant human-derived EphA4 protein, expressed by HEK293 , with tag free. The total length of EphA4 Protein, Human (HEK293) is 528 a.a., with molecular weight of 60-80 kDa.

The EphA4 protein is a receptor tyrosine kinase that interacts with membrane-bound ephrin ligands on adjacent cells, enabling bidirectional signaling. It can be activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands, such as EFNA1 and EFNB3. Activation of EphA4 modulates cell morphology and integrin-dependent cell adhesion by regulating the activity of Rac, Rap, and Rho GTPases. In the nervous system, EphA4 plays a crucial role in axonal guidance, including corticospinal projections and segregation of motor and sensory axons during neuromuscular circuit development. It also contributes to synaptic plasticity and is involved in repair after injury, preventing axonal regeneration. EphA4's promiscuity allows it to participate in various cell-cell signaling processes, including the development of the thymic epithelium. Furthermore, it regulates pillar cell separation in the cochlear organ of Corti by forming a complex with ADAM10 and CADH1, leading to the disruption of adherens junctions. EphA4 phosphorylates CAPRIN1, promoting the formation of a membraneless compartment.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

Tag Free

P54764 (V20-T547)

2043  [NCBI]

VTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRENQFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANST

60-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.