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  4. ERLIN1 Protein, Human (Cell-Free, His)

ERLIN1 Protein, Human (Cell-Free, His)

Cat. No.: HY-P702272
Handling Instructions

The ERLIN1 protein is an important component of the ERLIN1/ERLIN2 complex and plays a key role in mediating endoplasmic reticulum-associated degradation (ERAD) of the inositol 1,4,5-trisphosphate receptor (IP3R). In addition to participating in ERAD, ERLIN1 also regulates cellular cholesterol homeostasis by regulating the SREBP signaling pathway. ERLIN1 Protein, Human (Cell-Free, His) is the recombinant human-derived ERLIN1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ERLIN1 Protein, Human (Cell-Free, His) is 348 a.a., with molecular weight of 42.0 kDa.

For research use only. We do not sell to patients.

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Description

The ERLIN1 protein is an important component of the ERLIN1/ERLIN2 complex and plays a key role in mediating endoplasmic reticulum-associated degradation (ERAD) of the inositol 1,4,5-trisphosphate receptor (IP3R). In addition to participating in ERAD, ERLIN1 also regulates cellular cholesterol homeostasis by regulating the SREBP signaling pathway. ERLIN1 Protein, Human (Cell-Free, His) is the recombinant human-derived ERLIN1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ERLIN1 Protein, Human (Cell-Free, His) is 348 a.a., with molecular weight of 42.0 kDa.
Background

ERLIN1 is a crucial component of the ERLIN1/ERLIN2 complex, facilitating the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Its involvement in the regulation of cellular cholesterol homeostasis is notable, as it modulates the SREBP signaling pathway. ERLIN1 also exhibits an ability to bind cholesterol, potentially promoting the endoplasmic reticulum (ER) retention of the SCAP-SREBF complex, thereby impacting cholesterol-related cellular processes. In the context of microbial infection, ERLIN1 plays a dual role in hepatitis C virus (HCV) infection, being required early on to initiate RNA replication and later to support the production of infectious virus particles. This multifaceted functionality underscores ERLIN1's importance in cellular processes related to protein degradation, cholesterol regulation, and viral infection.
Species

Human
Source

E. coli Cell-free
Tag

N-10*His
Accession

O75477 (M1-G348)
Gene ID

10613
Molecular Construction
N-term
10*His
ERLIN1 (M1-G348)
Accession # O75477
C-term
Synonyms
Erlin-1; Endoplasmic reticulum lipid raft-associated protein 1; Protein KE04; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1; SPFH domain-containing protein 1
AA Sequence

MNMTQARVLVAAVVGLVAVLLYASIHKIEEGHLAVYYRGGALLTSPSGPGYHIMLPFITTFRSVQTTLQTDEVKNVPCGTSGGVMIYIDRIEVVNMLAPYAVFDIVRNYTADYDKTLIFNKIHHELNQFCSAHTLQEVYIELFDQIDENLKQALQKDLNLMAPGLTIQAVRVTKPKIPEAIRRNFELMEAEKTKLLIAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLAREKAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSSCALKYSDIRTGRESSLPSKEALEPSGENVIQNKESTG

Molecular Weight

42.0 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

ERLIN1 Protein, Human (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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