Fractalkine/CX3CL1 Protein, Mouse (HEK293, His)

The Fractalkine/CX3CL1 protein is a multifunctional chemokine that binds to CX3CR1 and the integrins ITGAV:ITGB3 and ITGA4:ITGB1. It regulates immune responses, inflammation, cell adhesion, and chemotaxis. Fractalkine/CX3CL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Fractalkine/CX3CL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Fractalkine/CX3CL1 Protein, Mouse (HEK293, His) is 313 a.a., with molecular weight of ~55-85 kDa.

Fractalkine/CX3CL1 protein functions as a versatile chemokine, serving as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling axis manifests distinct functions in various tissue compartments, including immune response modulation, inflammation regulation, cell adhesion, and chemotaxis. In the context of endothelial interactions, Fractalkine/CX3CL1 plays a pivotal role in regulating leukocyte adhesion and migration processes. Notably, it can activate integrins in a CX3CR1-dependent or CX3CR1-independent manner, binding to both the classical ligand-binding site (site 1) and a distinct site (site 2) on integrins. In the presence of CX3CR1, it activates integrins directly through site 1, while in the absence of CX3CR1, it binds to site 2, enhancing the binding of other integrin ligands to site 1. Additionally, the soluble form of Fractalkine/CX3CL1 demonstrates chemotactic properties for T-cells and monocytes, albeit not for neutrophils.

Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ this effect is 9.640 ng/mL, corresponding to a specific activity is 103734.440 U/mg.

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  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 9.640 ng/mL, corresponding to a specific activity is 103734.440 U/mg.

Mouse

HEK293

C-6*His

O35188 (Q25-R337)

20312  [NCBI]

QHLGMTKCEIMCDKMTSRIPVALLIRYQLNQESCGKRAIVLETTQHRRFCADPKEKWVQDAMKHLDHQAAALTKNGGKFEKRVDNVTPGITLATRGLSPSALTKPESATLEDLALELTTISQEARGTMGTSQEPPAAVTGSSLSTSEAQDAGLTAKPQSIGSFEAADISTTVWPSPAVYQSGSSSWAEEKATESPSTTAPSPQVSTTSPSTPEENVGSEGQPPWVQGQDLSPEKSLGSEEINPVHTDNFQERGPGNTVHPSVAPISSEETPSPELVASGSQAPKIEEPIHATADPQKLSVLITPVPDTQAATR

Approximately 55-85 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.