IL-34 Protein, Mouse (HEK293, His)

IL-34 protein is an important cytokine that promotes the proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immune responses and inflammatory processes by promoting the release of pro-inflammatory chemokines. IL-34 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-34 protein, expressed by HEK293 , with C-His labeled tag. The total length of IL-34 Protein, Mouse (HEK293, His) is 235 a.a., with molecular weight of 38-40 kDa.

Interleukin-34 (IL-34) is a cytokine with pivotal functions in innate immunity and inflammatory processes, as it promotes the proliferation, survival, and differentiation of monocytes and macrophages. Additionally, IL-34 plays a crucial role in the regulation of osteoclast proliferation and differentiation, influencing bone resorption. The cytokine's impact is mediated through its interaction with CSF1R, leading to the activation of downstream effectors and subsequent phosphorylation of MAPK1/ERK2 and MAPK3/ERK1. Structurally, IL-34 exists as a homodimer, and its intricate involvement in immune regulation and bone homeostasis highlights its significance in orchestrating cellular responses and maintaining tissue integrity.

1.Measured in a cell proliferation assay using M-NFS-60 mouse myeloid cells and the ED₅₀ is typically ≤200 ng/mL.
2.Measured by its ability to bind biotinylated recombinant mouse CSF1R in a functional ELISA.

Mouse

HEK293

C-His

Q8R1R4 (N21-P235)

76527  [NCBI]

NENLEIWTLTQDKECDLTGYLRGKLQYKNRLQYMKHYFPINYRIAVPYEGVLRVANITRLQKAHVSERELRYLWVLVSLNATESVMDVLLEGHPSWKYLQEVQTLLENVQRSLMDVEIGPHVEAVLSLLSTPGLSLKLVRPKALLDNCFRVMELLYCSCCKQSPILKWQDCELPRLHPHSPGSLMQCTATNVYPLSRQTPTSLPGSPSSSHGSLP

Approximately 38-40 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.