2. Recombinant Proteins
  3. Receptor Proteins Biotinylated Proteins
  4. Cell Adhesion Molecules (CAMs)
  5. Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His)

Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His)

Cat. No.: HY-P77547
Handling Instructions

FITC-Labeled Mesothelin Protein, especially in its membrane-anchored forms, potentially mediates cellular adhesion, implicating its role in cell interactions. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) actively enhances in vitro megakaryocyte colony formation, indicating its participation in fostering specialized blood cell colonies' development. Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-His labeled tag. The total length of Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His) is 286 a.a., with molecular weight of ~28.2 KDa.

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Description

FITC-Labeled Mesothelin Protein, especially in its membrane-anchored forms, potentially mediates cellular adhesion, implicating its role in cell interactions. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) actively enhances in vitro megakaryocyte colony formation, indicating its participation in fostering specialized blood cell colonies' development. Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-His labeled tag. The total length of Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His) is 286 a.a., with molecular weight of ~28.2 KDa.
Background

FITC-Labeled Mesothelin Protein, particularly in its membrane-anchored forms, exhibits a potential role in cellular adhesion, suggesting involvement in mediating interactions between cells. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) has been identified as a potent enhancer of megakaryocyte colony formation in vitro, indicating its active participation in fostering the development of these specialized blood cell colonies.
Species

Human
Source

HEK293
Tag

C-His
Accession

Q13421-2 (M1-R286)
Gene ID
Molecular Construction
N-term
Mesothelin (M1-R286)
Accession # Q13421-2
His
C-term
Synonyms
Megakaryocyte potentiating factor; mesothelin; CAK1; MPF; MSLN; SMR
Molecular Weight

Approximately 28.2 kDa.
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Mesothelin Protein, Human (Biotinylated, 286a.a, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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