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  5. MICAL2 Protein, Human (P.pastoris, His)

MICAL2 Protein, Human (P.pastoris, His)

Cat. No.: HY-P71756
Handling Instructions

The MICAL2 protein is a methionine monooxygenase that crucially promotes F-actin depolymerization by oxidizing actin residues "Met-44" and "Met-47" to form methionine sulfoxide. This modification prevents actin filaments from repolymerizing. MICAL2 Protein, Human (P.pastoris, His) is the recombinant human-derived MICAL2 protein, expressed by P. pastoris , with N-His labeled tag. The total length of MICAL2 Protein, Human (P.pastoris, His) is 495 a.a., with molecular weight of ~58.5 kDa.

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Description

The MICAL2 protein is a methionine monooxygenase that crucially promotes F-actin depolymerization by oxidizing actin residues "Met-44" and "Met-47" to form methionine sulfoxide. This modification prevents actin filaments from repolymerizing. MICAL2 Protein, Human (P.pastoris, His) is the recombinant human-derived MICAL2 protein, expressed by P. pastoris , with N-His labeled tag. The total length of MICAL2 Protein, Human (P.pastoris, His) is 495 a.a., with molecular weight of ~58.5 kDa.
Background

MICAL2, a methionine monooxygenase, plays a crucial role in promoting the depolymerization of F-actin by mediating the oxidation of residues 'Met-44' and 'Met-47' on actin, resulting in the formation of methionine-sulfoxide. This oxidative modification leads to actin filament disassembly, preventing repolymerization. Additionally, MICAL2 regulates the disassembly of branched actin networks by oxidizing ARP3B-containing ARP2/3 complexes, ultimately causing ARP3B dissociation from the network. Furthermore, MICAL2 serves as a key regulator in the SRF signaling pathway induced by nerve growth factor and serum. It mediates the oxidation and subsequent depolymerization of nuclear actin, enhancing the presence of MKL1/MRTF-A in the nucleus and facilitating SRF:MKL1/MRTF-A-dependent gene transcription. Notably, MICAL2 does not activate the SRF:MKL1/MRTF-A pathway through RhoA.
Species

Human
Source

P. pastoris
Tag

N-His
Accession

O94851 (1M-495H)
Gene ID
Molecular Construction
N-term
His
MICAL2 (1M-495H)
Accession # O94851
C-term
Synonyms
MICAL2; KIAA0750; MICAL2PV1; MICAL2PV2
AA Sequence

MGENEDEKQAQAGQVFENFVQASTCKGTLQAFNILTRHLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGKSCTNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDSFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPTDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDCTHYFVMTAKKQSLLDKGVIINDYIDTEMLLCAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTCMYASENAALVRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWNQGTPPLELLAERESLYRLLPQTTPENINKNFEQYTLDPGTRYPNLNSHCVRPHQVKHLYITKELEH

Molecular Weight

Approximately 58.5 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MICAL2 Protein, Human (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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