MSP/MST1 Protein, Mouse (C677A, HEK293)

The MSP/MST1 protein, encoded by this gene, is predicted to have receptor tyrosine kinase binding activity. It is involved in various processes, including embryo implantation, mammary duct growth, and regulation of mammary gland cell proliferation. The protein is mainly found in the vacuole and expressed in tissues like the esophagus, liver, pharynx, and stomach. Notably, the gene shows liver-biased expression in both adult and embryonic stages. MSP/MST1 Protein, Mouse (C677A, HEK293) is the recombinant mouse-derived MSP/MST1 protein, expressed by HEK293 , with tag free. and C677A mutation. The total length of MSP/MST1 Protein, Mouse (C677A, HEK293) is 698 a.a., with molecular weight of 92 & 60.5 & 32-36 kDa, respectively.

This gene, encoding the MSP/MST1 protein, is predicted to possess receptor tyrosine kinase binding activity. It plays a role upstream in various processes, including embryo implantation, mammary duct terminal end bud growth, and positive regulation of mammary gland epithelial cell proliferation. The protein is primarily located in the vacuole and is expressed in tissues such as esophagus epithelium, liver, liver parenchyma, pharynx epithelium, and stomach proventricular region epithelium. Notably, the gene exhibits biased expression in the liver of both adult and embryonic stages in the reference dataset.

Measured by its ability to inhibit the proliferation of Hela cell. The ED₅₀ for this effect is 4.080 ng/ml, corresponding to a specific activity is 2.45×10^5 units/mg.

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  Measured by its ability to inhibit the proliferation of Hela cell. The ED₅₀ for this effect  is 4.080 ng/mL, corresponding to a specific activity is 2.45×10⁵ units/mg.

Mouse

HEK293

Tag Free

NP_032269 (Q19-E716, C677A)

15235  [NCBI]

QRSPLNDFQLFRGTELRNLLHTAVPGPWQEDVADAEECARRCGPLLDCRAFHYNMSSHGCQLLPWTQHSLHTQLYHSSLCHLFQKKDYVRTCIMDNGVSYRGTVARTAGGLPCQAWSRRFPNDHKYTPTPKNGLEENFCRNPDGDPRGPWCYTTNRSVRFQSCGIKTCREAVCVLCNGEDYRGEVDVTESGRECQRWDLQHPHSHPFQPEKFLDKDLKDNYCRNPDGSERPWCYTTDPNVEREFCDLPSCGPNLPPTVKGSKSQRRNKGKALNCFRGKGEDYRGTTNTTSAGVPCQRWDAQSPHQHRFVPEKYACKDLRENFCRNPDGSEAPWCFTSRPGLRMAFCHQIPRCTEELVPEGCYHGSGEQYRGSVSKTRKGVQCQHWSSETPHKPQFTPTSAPQAGLEANFCRNPDGDSHGPWCYTLDPDILFDYCALQRCDDDQPPSILDPPDQVVFEKCGKRVDKSNKLRVVGGHPGNSPWTVSLRNRQGQHFCGGSLVKEQWVLTARQCIWSCHEPLTGYEVWLGTINQNPQPGEANLQRVPVAKAVCGPAGSQLVLLKLERPVILNHHVALICLPPEQYVVPPGTKCEIAGWGESIGTSNNTVLHVASMNVISNQECNTKYRGHIQESEICTQGLVVPVGACEGDYGGPLACYTHDAWVLQGLIIPNRVCARPRWPAIFTRVSVFVDWINKVMQLE

92 & 60.5 & 32-36 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.