NEK6 Protein, Human (Sf9, GST)

The NEK6 protein is a key kinase essential for driving mitotic cell cycle progression, ensuring chromosome segregation, robust spindle formation, and cytokinesis. It phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histone H1/H3, affecting a variety of cellular processes. NEK6 Protein, Human (Sf9, GST) is the recombinant human-derived NEK6 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of NEK6 Protein, Human (Sf9, GST) is 312 a.a., .

NEK6 protein, a pivotal kinase, assumes a crucial role in driving mitotic cell cycle progression with indispensable functions in chromosome segregation at the metaphase-anaphase transition, robust mitotic spindle formation, and cytokinesis. Its substrate spectrum includes ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3, and histones H1 and H3, reflecting its versatile impact on various cellular processes. NEK6's phosphorylation of KIF11 promotes mitotic spindle formation, and it actively participates in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of NEK6 activity leads to apoptosis, underlining its essential role in cell survival. Intriguingly, NEK6 may contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. Moreover, its phosphorylation of EML4 at 'Ser-144' promotes dissociation from microtubules during mitosis, a critical step for efficient chromosome congression. NEK6's multifaceted functions underscore its significance in orchestrating key events during mitotic progression and its potential implications in cancer-related processes.

Human

Sf9 insect cells

N-GST

Q9HC98 (A2-T313)

10783

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.