PLA2G7 Protein, Human (HEK293, His)

PLA2G7 Protein is a lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress responses. It is also a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. PLA2G7 can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. PLA2G7 Protein, Human (HEK293, His) is the recombinant human-derived PLA2G7 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PLA2G7 Protein, Human (HEK293, His) is 420 a.a., with molecular weight of ~57 kDa.

Platelet-activating factor acetylhydrolase (PLA2G7) is a lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress responses. At the lipid-aqueous interface, PLA2G7 hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids, that specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. It also hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. In addition, PLA2G7 hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. As part of high-density lipoprotein (HDL) particles, PLA2G7 can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. PLA2G7 catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage^{[1][2][3][4][5]}.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

AAH38452.1 (F22-N441)

7941  [NCBI]

FDWQYINPVAHMKSSAWVNKIQVLMAAASFGQTKIPRGNGPYSVGCTDLMFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVIQTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKCYSPDKERKMITIRGSVHQNFADFTFATGKIIGHMLKLKGDIDSNAAIDLSNKASLAFLQKHLGLHKDFDQWDCLIEGDDENLIPGTNINTTNQHIMLQNSSGIEKYN

Approximately 57 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM NaAc, 150 mM NaCl, 10% Glycerol, pH 5.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.