PRKD1 Protein, Human (Sf9)

PRKD1 is a serine/threonine protein kinase that controls multiple cellular functions and transduces transient diacylglycerol (DAG) signals downstream of PKC. PRKD1 Protein, Human (Sf9) is the recombinant human-derived PRKD1 protein, expressed by Sf9 insect cells , with tag free. The total length of PRKD1 Protein, Human (Sf9) is 911 a.a., .

PRKD1, a serine/threonine-protein kinase, orchestrates a myriad of cellular functions by converting transient diacylglycerol (DAG) signals into sustained physiological effects downstream of PKC. Involved in diverse pathways, PRKD1 regulates MAPK8/JNK1 and Ras signaling, maintains Golgi membrane integrity, promotes cell survival through NF-kappa-B activation, influences cell migration, mediates cell differentiation by facilitating HDAC7 nuclear export, regulates cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays roles in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis, and flagellin-stimulated inflammatory responses. Notably, PRKD1 phosphorylates the epidermal growth factor receptor (EGFR), suppressing EGF-induced MAPK8/JNK1 activation and subsequent JUN phosphorylation. It also phosphorylates RIN1, affecting its binding to 14-3-3 proteins and modulating competition with RAF1 for binding to GTP-bound Ras proteins. Furthermore, PRKD1 participates in Golgi membrane trafficking, impacting protein transport along the secretory pathway and orchestrating vesicle fission in the trans-Golgi network (TGN). The kinase's multifaceted involvement extends to angiogenesis, osteoblast differentiation, and cardiac hypertrophy, demonstrating its critical regulatory roles in various cellular processes.

Human

Sf9 insect cells

Tag Free

Q15139 (S2-L912)

/

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.