1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. TKT Protein, Human

TKT Protein, Human

Cat. No.: HY-P701864
Handling Instructions

TKT proteins are essential for catalyzing the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor. This process is facilitated by the formation of a covalent intermediate with the cofactor thiamine pyrophosphate. TKT Protein, Human is the recombinant human-derived TKT protein, expressed by E. coli , with tag free. The total length of TKT Protein, Human is 622 a.a., .

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

TKT proteins are essential for catalyzing the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor. This process is facilitated by the formation of a covalent intermediate with the cofactor thiamine pyrophosphate. TKT Protein, Human is the recombinant human-derived TKT protein, expressed by E. coli , with tag free. The total length of TKT Protein, Human is 622 a.a., .

Background

TKT protein is essential for catalyzing the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor. This process is facilitated by the formation of a covalent intermediate with the cofactor thiamine pyrophosphate. The enzymatic activity of TKT protein plays a critical role in various metabolic pathways, including the pentose phosphate pathway and the Calvin cycle. The transfer of the ketol group is a key step in these pathways, enabling the conversion of sugars and the generation of important intermediates for energy production and biosynthesis. TKT protein's function is crucial for maintaining metabolic flux and contributing to the overall balance of carbohydrate metabolism in the cell.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P29401 (E2-A623)

Gene ID

7086

Molecular Construction
N-term
TKT (E2-A623)
Accession # P29401
C-term
Synonyms
TKT; Transketolase; TK
Purity

Greater than 85% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

TKT Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TKT Protein, Human
Cat. No.:
HY-P701864
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