TrkB Protein, Rat (HEK293, C-His)

TrkB, a pivotal receptor tyrosine kinase, orchestrates diverse neuronal processes in the central and peripheral nervous systems. Serving as a receptor for BDNF and NTF4, it modulates survival through NTF3. Upon ligand binding, TrkB undergoes activation, initiating downstream cascades. It recruits effectors like SHC1, FRS2, SH2B1, SH2B2, and PLCG1, regulating pathways for differentiation, growth, survival, and synaptic plasticity. TrkB's role in learning involves short-term synaptic function, long-term potentiation, and PLCG1-mediated suppression of anoikis. Additionally, it contributes to neurotrophin-dependent calcium signaling in glial cells. TrkB Protein, Rat (HEK293, C-His) is the recombinant rat-derived TrkB protein, expressed by HEK293, with C-6*His labeled tag. The total length of TrkB Protein, Rat (HEK293, C-His) is 398 a.a., with molecular weight of ~62-85 kDa.

TrkB, a receptor tyrosine kinase, intricately orchestrates the development and maturation of both the central and peripheral nervous systems, exerting regulatory control over various neuronal processes, including survival, proliferation, migration, differentiation, and synapse formation. Functioning as a receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4, it can alternatively bind NTF3/neurotrophin-3, modulating neuron survival through NTRK2. Upon ligand binding, TrkB undergoes homodimerization, autophosphorylation, and activation, initiating a cascade of downstream events. It recruits, phosphorylates, and activates diverse effectors such as SHC1, FRS2, SH2B1, SH2B2, and PLCG1, which coordinate distinct signaling pathways, including the GRB2-Ras-MAPK cascade for neuronal differentiation, the Ras-PI3 kinase-AKT1 pathway for growth and survival, and PLCG1-regulated pathways for synaptic plasticity. TrkB's involvement in learning and memory encompasses the regulation of short-term synaptic function and long-term potentiation. Additionally, through PLCG1, it activates NF-Kappa-B and transcription of survival-related genes, enabling the suppression of anoikis—a form of apoptosis resulting from the loss of cell-matrix interactions. Notably, TrkB may also contribute to neurotrophin-dependent calcium signaling in glial cells.

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED₅₀ this effect is 0.3638 μg/mL, corresponding to a specific activity is 2.75×10^3 units/mg.

Rat

HEK293

C-6*His

Q63604-2 (C32-H429)

25054

CPMSCKCSTTRIWCTEPSPGIVAFPRLEPNSIDPENITEILIANQKRLEIINEDDVEAYVGLKNLTIVDSGLKFVAYKAFLKNGNLRHINFTRNKLTSLSRRHFRHLDLSDLILTGNPFTCSCDIMWLKTLQETKSSPDTQDLYCLNESSKNTPLANLQIPNCGLPSARLAAPNLTVEEGKSVTISCSVGGDPLPTLYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVRGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLMAKNEYGKDERQISAHFMGRPGVDYETNPNYPEVLYEDWTTPTDIGDTTNKSNEIPSTDVADQTNREH

Approximately 62-85 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.The protein migrates as 62-85 kDa under reducing condition (SDS-PAGE) due to glycosylation.

Lyophilized powder.

Lyophilized from a 0.2 μm solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.