2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. USP28-IN-4

USP28-IN-4 is a USP28 inhibitor (IC50=0.04 μM) with high selectivity over USP2, USP7, USP8, USP9x, UCHL3 and UCHL5. USP28-IN-4 shows cytotoxicity against cancer cells, down-regulates the cellular level of c-Myc through ubiquitin-proteasome system. USP28-IN-4 also decreases the ankyrase-1/2 level in vitro. USP28-IN-4 enhance the sensitivity of colorectal cancer cells to Regorafenib (HY-10331).

For research use only. We do not sell to patients.

USP28-IN-4 Chemical Structure

USP28-IN-4 Chemical Structure

CAS No. : 2931509-15-6

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Based on 1 publication(s) in Google Scholar

USP28-IN-4 Related Antibodies

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Description

USP28-IN-4 is a USP28 inhibitor (IC50=0.04 μM) with high selectivity over USP2, USP7, USP8, USP9x, UCHL3 and UCHL5. USP28-IN-4 shows cytotoxicity against cancer cells, down-regulates the cellular level of c-Myc through ubiquitin-proteasome system. USP28-IN-4 also decreases the ankyrase-1/2 level in vitro. USP28-IN-4 enhance the sensitivity of colorectal cancer cells to Regorafenib (HY-10331)[1].

IC50 & Target[1]

USP28

0.04 μM (IC50)

In Vitro

USP28-IN-4 (compound 9p) (12.5 μM, 15 μM; 3 d) inhibits colony formation of human colorectal cancer cells HCT116 (15 μM) and Ls174T (12.5 μM)[1].
USP28-IN-4 (20-80 μM; 24 h) down-regulates the level of c-Myc by enhancing its degradation via ubiquitin-proteasome system (UPS)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

USP28-IN-4 Related Antibodies

Western Blot Analysis[1]

Cell Line: Human colorectal cancer cells HCT116 and Ls174T
Concentration: 20 μM, 30 μM, 50 μM, and 60 μM, for Ls174T; 30 μM, 50 μM, 60 μM and 80 μM for HCT116
Incubation Time: 24 h
Result: Dose-dependently down-regulated the cellular level of c-Myc.
Molecular Weight

461.36

Formula

C22H18Cl2N2O3S
CAS No.
Appearance

Solid

Color

Light yellow to brown

SMILES

CS(=O)(C1=CC=C(C(Cl)=C1)C(NC2=CC=C(C(C3=CC4=C(CNC4)C=C3)=C2)Cl)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (54.19 mM; ultrasonic and adjust pH to 6 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1675 mL 10.8375 mL 21.6750 mL
5 mM 0.4335 mL 2.1675 mL 4.3350 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1675 mL 10.8375 mL 21.6750 mL 54.1876 mL
5 mM 0.4335 mL 2.1675 mL 4.3350 mL 10.8375 mL
10 mM 0.2168 mL 1.0838 mL 2.1675 mL 5.4188 mL
15 mM 0.1445 mL 0.7225 mL 1.4450 mL 3.6125 mL
20 mM 0.1084 mL 0.5419 mL 1.0838 mL 2.7094 mL
25 mM 0.0867 mL 0.4335 mL 0.8670 mL 2.1675 mL
30 mM 0.0723 mL 0.3613 mL 0.7225 mL 1.8063 mL
40 mM 0.0542 mL 0.2709 mL 0.5419 mL 1.3547 mL
50 mM 0.0434 mL 0.2168 mL 0.4335 mL 1.0838 mL
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USP28-IN-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP28-IN-42931509-15-6DeubiquitinaseDUBsUSP28USP2USP7USP8USP9xUCHL3UCHL5. USP28-IN-2c-Mycubiquitin-proteasome systemankyrase-1/2colorectal cancer cellInhibitorinhibitorinhibit

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