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  4. DiMethyl-Histone H3 Antibody (YA783)

DiMethyl-Histone H3 Antibody (YA783)

Cat. No.: HY-P80643
User Guide for Antibodies

DiMethyl-Histone H3 Antibody (YA783) is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to DiMethyl-Histone H3 (Lys27) (5F6). It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size Price Stock
10 μL $70 Ask For Quote & Lead Time
50 μL $185 Ask For Quote & Lead Time
100 μL $300 Ask For Quote & Lead Time

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Description

DiMethyl-Histone H3 Antibody (YA783) is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to DiMethyl-Histone H3 (Lys27) (5F6). It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.

Background

DiMethyl-Histone H3 (Lys27) (5F6): Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Tag

Free

Gene ID

8350

Synonyms
H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Molecular Weight

Predicted band size: 15 kDa

Purity

affinity purified

Conjugation

Non-conjugated

Modification

Methylated

Research Field

Epigenetics and Nuclear Signaling

Product Categories

Primary Antibody; Mouse Monoclonal Antibody

Clonality

Monoclonal

Host

Mouse

Reactivity

Human, Mouse, Rat

SwissProt ID

P68431

Dilution Ratio

WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;IP: 1:20

Application

WB, ICC/IF, IP

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG1

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Histone H3 (Di Methyl Lys27).The exact sequence is proprietary to MCE.

Documentation

DiMethyl-Histone H3 Antibody (YA783) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
DiMethyl-Histone H3 Antibody (YA783)
Cat. No.:
HY-P80643
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