Butaprost

Cat. No.: HY-100448A Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Butaprost is a selective prostaglandin E receptor (EP2) agonist with an EC₅₀ of 33 nM and a K_i of 2.4 μM for murine EP2 receptor. Butaprost is less activity against murine EP1, EP3 and EP4 receptors. Butaprost attenuates fibrosis by hampering TGF-β/Smad2 signalling.

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Butaprost Chemical Structure

CAS No. : 69685-22-9

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1 mg (12.24 mM * 200 μL in Methyl acetate)	USD 390	In-stock	Estimated Time of Arrival: December 31
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5 mg (12.24 mM * 1 mL in Methyl acetate)	USD 1168	In-stock	Estimated Time of Arrival: December 31

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Description

In Vitro

Butaprost (1-100 nM; 0.5-24 hours) induces about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells in a dose- and time-dependent. Butaprost upregulated Nur77 gene expression through the PKC pathway^[1].
Butaprost (50 μM; 24 hours) reduces TGF-β-induced fibronectin (FN) expression, Smad2 phosphorylation and epithelial-mesenchymal transition in Madin-Darby Canine Kidney (MDCK) cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	HEK 293/EBNA cells stably expressing the human EP2 receptor (hEP2-HEK 293/EBNA cells)
Concentration:	1 nM, 10 nM, 100 nM
Incubation Time:	0.5 hours, 1 hours, 6 hours, 24 hours
Result:	Induced about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells.

In Vivo

Butaprost (1-4 mg/kg; intraperitoneal injection; twice daily; for 7 days) treatment attenuates the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery, as illustrated by a reduction in the gene and protein expression of α-smooth muscle actin, fibronectin and collagen 1A1^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice (8 weeks of age; 21 g) bearing unilateral ureteral obstruction surgery^[2]
Dosage:	1 mg/kg, 2 mg/kg, 4 mg/kg
Administration:	Intraperitoneal injection; twice daily; for 7 days
Result:	Attenuated the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery.

Molecular Weight

408.57

Formula

C₂₄H₄₀O₅

CAS No.

69685-22-9

Unlabeled CAS

Appearance

Liquid

Color

Colorless to light yellow

SMILES

COC(CCCCCC[C@@H](C(C[C@H]1O)=O)[C@H]1/C=C/C[C@@H](C2(CCC2)CCC)O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Purity & Documentation

Purity: ≥99.0%

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Data Sheet (274 KB) SDS (251 KB)

COA (252 KB)

Handling Instructions (2659 KB)

References

[1]. Yanbin Liang, et al. Upregulation of orphan nuclear receptor Nur77 following PGF(2alpha), Bimatoprost, and Butaprost treatments. Essential role of a protein kinase C pathway involved in EP(2) receptor activated Nur77 gene transcription. Br J Pharmacol. 20 [Content Brief]

[2]. Michael Schou Jensen, et al. Activation of the prostaglandin E 2 EP 2 receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices. Acta Physiol (Oxf). 2019 Sep;227(1):e13291. [Content Brief]

[3]. K Tani, et al. Design and synthesis of a highly selective EP2-receptor agonist. Bioorg Med Chem Lett. 2001 Aug 6;11(15):2025-8. [Content Brief]

Butaprost Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Butaprost69685-22-9Prostaglandin ReceptorTGF-beta/SmadTransforming growth factor betaNur77EP2TGF-βSmad2fibronectinfibrogenesisInhibitorinhibitorinhibit

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