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  4. Fura-2 pentapotassium

Fura-2 pentapotassium is a scaled fluorescent dye that can be used for intracellular calcium imaging with the Kd value of 140 nM. Fura-2 pentapotassium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca2+ level.

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Fura-2 pentapotassium Chemical Structure

Fura-2 pentapotassium Chemical Structure

CAS No. : 113694-64-7

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500 μg USD 132 In-stock

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Based on 1 publication(s) in Google Scholar

Fura-2 pentapotassium Related Antibodies

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Description

Fura-2 pentapotassium is a scaled fluorescent dye that can be used for intracellular calcium imaging with the Kd value of 140 nM. Fura-2 pentapotassium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca2+ level[1][2].

In Vitro

Fura-2 pentapotassium (10 μM, 2 min) can be used to detect changes in the concentration of calcium ions in neutrophils of heparinized peripheral blood[1].
Fura-2 pentapotassium (10 μM, 5 min) can be used as a calcium probe to assess the distribution of calcium ions within heart mitochondria without affecting the activity of it in rat[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fura-2 pentapotassium Related Antibodies
Molecular Weight

831.99

Formula

C29H22K5N3O14
CAS No.
Unlabeled CAS

Appearance

Solid

Color

White to off-white

Emission (Em)

540
Excitation (Ex)

510
SMILES

O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(O[K])=O)CC(O[K])=O)=C(N(CC(O[K])=O)CC(O[K])=O)C=C3O2)O1)O[K]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

1M NaOH : 10 mg/mL (12.02 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2019 mL 6.0097 mL 12.0194 mL
5 mM 0.2404 mL 1.2019 mL 2.4039 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥96.0%

COA (255 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
1M NaOH 1 mM 1.2019 mL 6.0097 mL 12.0194 mL 30.0484 mL
5 mM 0.2404 mL 1.2019 mL 2.4039 mL 6.0097 mL
10 mM 0.1202 mL 0.6010 mL 1.2019 mL 3.0048 mL
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Fura-2 pentapotassium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fura-2 pentapotassium113694-64-7Fluorescent DyeCa2+ Fluorescent IndicatorInhibitorinhibitorinhibit

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