1. Apoptosis
  2. DAPK
  3. HS38

HS38 is a potent, selective, and ATP-competitive inhibitor of death-associated protein kinase 1 (DAPK1) and zipper-interacting protein kinase (ZIPK, also called DAPK3), with Kds of 300 nM and 280 nM, respectively. HS38 is also a PIM3 inhibitor with an IC50 of 200 nM. HS38 can be used for the research of smooth muscle related disorders.

For research use only. We do not sell to patients.

HS38 Chemical Structure

HS38 Chemical Structure

CAS No. : 1030203-81-6

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
5 mg USD 60 In-stock
10 mg USD 85 In-stock
25 mg USD 170 In-stock
50 mg USD 270 In-stock
100 mg USD 440 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE HS38

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HS38 is a potent, selective, and ATP-competitive inhibitor of death-associated protein kinase 1 (DAPK1) and zipper-interacting protein kinase (ZIPK, also called DAPK3), with Kds of 300 nM and 280 nM, respectively. HS38 is also a PIM3 inhibitor with an IC50 of 200 nM. HS38 can be used for the research of smooth muscle related disorders[1].

IC50 & Target

Kd: 300 nM (DAPK1), 79 nM (DAPK2), 280 nM (ZIPK), IC50: 200 nM (DAPK1), 200 nM (PIM3)[1]

In Vitro

HS38 displays high affinity toward DAPK2, with a Kd of 79 nM. DAPK2 is not implicated in smooth muscle contractility[1].
HS38 significantly reduces relative RLC20 phosphorylation in both the basal and sphingosine 1-phosphate (S1P) activated states in human aortic SM cells[1].
HS38 reduces contractile forces generated by intact mouse aorta in aortic tissue[1].
HS38 reduces the contractile force, RLC20 phosphorylation, and MYPT1 phosphorylation in Ca2+-sensitized rabbit ileum[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: CA-VSMCs
Concentration: 10 µM
Incubation Time: 40 minutes
Result: Reduced relative RLC20 phosphorylation in both the basal and S1P activated states.
Molecular Weight

349.80

Formula

C14H12ClN5O2S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(SC1=NC(N(C2=CC=CC(Cl)=C2)N=C3)=C3C(N1)=O)C(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 7.14 mg/mL (20.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8588 mL 14.2939 mL 28.5878 mL
5 mM 0.5718 mL 2.8588 mL 5.7176 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8588 mL 14.2939 mL 28.5878 mL 71.4694 mL
5 mM 0.5718 mL 2.8588 mL 5.7176 mL 14.2939 mL
10 mM 0.2859 mL 1.4294 mL 2.8588 mL 7.1469 mL
15 mM 0.1906 mL 0.9529 mL 1.9059 mL 4.7646 mL
20 mM 0.1429 mL 0.7147 mL 1.4294 mL 3.5735 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

HS38 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
HS38
Cat. No.:
HY-15847
Quantity:
MCE Japan Authorized Agent: