2. Academic Validation
  3. Gingerenone A Induces Antiproliferation and Senescence of Breast Cancer Cells

Gingerenone A Induces Antiproliferation and Senescence of Breast Cancer Cells

  • Antioxidants (Basel). 2022 Mar 19;11(3):587. doi: 10.3390/antiox11030587.
Tzu-Jung Yu 1,  Jen-Yang Tang 2 3,  Jun-Ping Shiau 4 5,  Ming-Feng Hou 4 5 6,  Chia-Hung Yen 1,  Fu Ou-Yang 4 5,  Chung-Yi Chen 7,  Hsueh-Wei Chang 6 8 9
Affiliations

Affiliations

  • 1 Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
  • 2 School of Post-Baccalaureate Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
  • 3 Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.
  • 4 Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.
  • 5 Division of Breast Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.
  • 6 Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
  • 7 Department of Nutrition and Health Sciences, School of Medical and Health Sciences, Fooyin University, Kaohsiung 83102, Taiwan.
  • 8 Center for Cancer Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
  • 9 Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
PMID: 35326237 DOI: 10.3390/antiox11030587
Abstract

Ginger is a popular spice and consists of several bioactive antioxidant compounds. Gingerenone A (Gin A), a novel compound isolated from Zingiber officinale, is rarely investigated for its anti-breast-cancer properties. Some ginger extracts have been reported to initiate senescence, an Anticancer strategy. However, the Anticancer effects of Gin A on breast Cancer cells remain unclear. The present study aims to assess the modulating impact of Gin A acting on proliferation and senescence to breast Cancer cells. Gin A diminished the cellular ATP content and decreased the cell viability of the MTS assay in several breast Cancer cell lines. It also showed a delayed G2/M response to breast Cancer cells (MCF7 and MDA-MB-231). N-acetylcysteine (NAC), an oxidative stress inhibitor, can revert these responses of antiproliferation and G2/M delay. The oxidative stress and senescence responses of Gin A were further validated by increasing Reactive Oxygen Species, mitochondrial superoxide, and β-galactosidase activity, which were reverted by NAC. Gin A also upregulated senescence-associated gene expressions. In addition to oxidative stress, Gin A also induced DNA damage responses by increasing γH2AX level and foci and generating 8-hydroxyl-2'-deoxyguanosine in breast Cancer cells, which were reverted by NAC. Therefore, Gin A promotes antiproliferation and senescence of breast Cancer cells induced by oxidative stress.

Keywords

DNA damage; breast cancer; ginger; oxidative stress; senescence.

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