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  4. N-Hydroxysuccinimide

N-Hydroxysuccinimide  (Synonyms: HOSu; 1-Hydroxy-2,5-pyrrolidinedione)

Cat. No.: HY-Y0623 Purity: 97.86%
COA Handling Instructions

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N-Hydroxysuccinimide (HOSu; 1-Hydroxy-2,5-pyrrolidinedione) is an activated ester commonly used in bioconjugation technologies such as protein labeling with fluorescent dyes and enzymes, chromatography supports, beads, nanoparticles, and microarrays Surface activation of glass slides, and chemically synthesized peptides.

For research use only. We do not sell to patients.

N-Hydroxysuccinimide Chemical Structure

N-Hydroxysuccinimide Chemical Structure

CAS No. : 6066-82-6

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Based on 1 publication(s) in Google Scholar

N-Hydroxysuccinimide Related Antibodies

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Description

N-Hydroxysuccinimide (HOSu; 1-Hydroxy-2,5-pyrrolidinedione) is an activated ester commonly used in bioconjugation technologies such as protein labeling with fluorescent dyes and enzymes, chromatography supports, beads, nanoparticles, and microarrays Surface activation of glass slides, and chemically synthesized peptides[1].

Molecular Weight

115.09

Formula

C4H5NO3
CAS No.
Unlabeled CAS

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1O)CCC1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (868.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.6889 mL 43.4443 mL 86.8885 mL
5 mM 1.7378 mL 8.6889 mL 17.3777 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.76%

COA (251 KB) HNMR (114 KB) RP-HPLC (226 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 8.6889 mL 43.4443 mL 86.8885 mL 217.2213 mL
5 mM 1.7378 mL 8.6889 mL 17.3777 mL 43.4443 mL
10 mM 0.8689 mL 4.3444 mL 8.6889 mL 21.7221 mL
15 mM 0.5793 mL 2.8963 mL 5.7926 mL 14.4814 mL
20 mM 0.4344 mL 2.1722 mL 4.3444 mL 10.8611 mL
25 mM 0.3476 mL 1.7378 mL 3.4755 mL 8.6889 mL
30 mM 0.2896 mL 1.4481 mL 2.8963 mL 7.2407 mL
40 mM 0.2172 mL 1.0861 mL 2.1722 mL 5.4305 mL
50 mM 0.1738 mL 0.8689 mL 1.7378 mL 4.3444 mL
60 mM 0.1448 mL 0.7241 mL 1.4481 mL 3.6204 mL
80 mM 0.1086 mL 0.5431 mL 1.0861 mL 2.7153 mL
100 mM 0.0869 mL 0.4344 mL 0.8689 mL 2.1722 mL
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N-Hydroxysuccinimide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Hydroxysuccinimide6066-82-6HOSu 1-Hydroxy-2,5-pyrrolidinedioneBiochemical Assay ReagentsInhibitorinhibitorinhibit

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