Autophagy assays are techniques used to evaluate the autophagy process. This includes detecting the expression of autophagosomes, autophagy-related proteins, and their activities. Common methods include observation under a light microscope, Western blot, fluorescent staining, etc. Autophagy is an important physiological process in which cells decompose and recycle organelles and proteins. It is involved in regulating cell survival and death. Autophagy assays are widely used in disease research, drug screening, and biomedical research.