Shuttle box experiment (TDPA, Two-Day Passive Avoidance) is a classic learning and memory behavioral experiment, commonly used in mouse or rat models. In experiments, animals are trained to avoid situations associated with electric shock stimulation. On the first day, animals learn to avoid a specific environment after being exposed to a conditioned stimulus (such as an electric shock) during the learning phase. The next day, a testing phase assessed the animals' short-term memory by observing whether they maintained their avoidance behavior. TDPA experiments are commonly used to study learning, memory, cognitive function, and cognitive deficits in neurological disease models.