TMED1 Protein, Mouse (HEK293, Fc)

TMED1, or Transmembrane p24 trafficking protein 1, is a member of the EMP24/GP25L family. TMED1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TMED1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TMED1 Protein, Mouse (HEK293, Fc) is 170 a.a., with molecular weight of ~56-60 KDa.

TMED1 Protein belongs to the TMED (transmembrane emp24 domain-containing) protein family, which is involved in the vesicular trafficking of proteins. TMED1 Protein was identified by its interaction with interleukin 1 receptor-like 1 (IL1RL1) and may play a role in innate immunity. TMED1 Protein lacks any similarity to other interleukin 1 ligands.

Measured by its binding ability in a functional ELISA. Immobilized Mouse TMED1 at 0.5 μg/mL (100 μL/well) can bind Human ST2. The ED₅₀ for this effect is 2.39 μg/mL.

Mouse

HEK293

C-hFc

B2RRM5/NP_034874.2 (G25-N194)

17083  [NCBI]

GPPPIQDGEFTFLLPAGRKQCFYQSAPANASLETEYQVIGGAGLDVDFTLESPQGVLLVSESRKADGVHTVEPTEAGDYRLCFDNSFSTISEKLVFFELIFDSFQDEEEVEGWAEAVEPEEMLDVKMEDIKESIETMRTRLERSIQMLTLLRAFEARDRNLQEDNLERVN

Approximately 56-60 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.