2. Neuronal Signaling Stem Cell/Wnt Metabolic Enzyme/Protease Apoptosis
  3. γ-secretase Proteasome Apoptosis
  4. Z-LLNle-CHO

Z-LLNle-CHO  (Synonyms: Z-Leu-Leu-Nle-CHO; GSII)

Cat. No.: HY-120234 Purity: 97.61%
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Z-LLNle-CHO (Z-Leu-Leu-Nle-CHO) is a γ-secretase inhibitor I. Z-LLNle-CHO induces caspase and ROS-dependent apoptosis by blocking the Akt-mediated pro-survival pathway. Z-LLNle-CHO can be used in cancer research, such as breast cancer and leukaemia.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-LLNle-CHO Chemical Structure

Z-LLNle-CHO Chemical Structure

CAS No. : 133407-83-7

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1 mg USD 95 In-stock
5 mg USD 240 In-stock
10 mg USD 360 In-stock
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Based on 1 publication(s) in Google Scholar

Z-LLNle-CHO Related Antibodies

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Description

Z-LLNle-CHO (Z-Leu-Leu-Nle-CHO) is a γ-secretase inhibitor I. Z-LLNle-CHO induces caspase and ROS-dependent apoptosis by blocking the Akt-mediated pro-survival pathway. Z-LLNle-CHO can be used in cancer research, such as breast cancer and leukaemia[1][2].

In Vitro

Z-LLNle-CHO (0-5 µM or 0-3 µM; 72 h) results in a dose-dependent decrease in cell viability/proliferation in six breast cancer cell lines[1].
Z-LLNle-CHO shows proteasome inhibitory activity, which contributes to cytotoxicity to MCF-7 cells[1].
Z-LLNle-CHO blocks Akt-mediated pro-survival pathways and induces caspase- and ROS-dependent cell apoptosis in Nalm6 and 697 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Z-LLNle-CHO Related Antibodies

Cell Viability Assay[1]

Cell Line: MCF-7, BT474, T47D, MDA-MB-231, SKBR3, and MDA-MB-468 cells
Concentration: 0-5 µM (for MCF-7); 0-3 µM
Incubation Time: 72 h
Result: Inhibited MCF-7, BT474, T47D, MDA-MB-231, SKBR3, and MDA-MB-468 cells with ED50 values of 3.25, 2.5, 2.4, 1.8, 1.6, and 1.4 μM, respectively.

Apoptosis Analysis[2]

Cell Line: Precursor-B ALL cells
Concentration: 0-2.5 µM
Incubation Time: 18-24 h
Result: Induced cell apoptosis.
In Vivo

Z-LLNle-CHO (5 mg/kg; s.c.; single daily for 12 days) inhibits engraftment of B-lymphoblasts in precursor-B ALL xenograft model[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female SCID/NOD mice (6-8-week-old; precursor-B ALL xenograft model)[2].
Dosage: 5 mg/kg
Administration: Subcutaneous injection; single daily for 12 days
Result: Delayed or prevented engraftment of B-lymphoblasts in 50% of the animals comprising the experimental group.
Molecular Weight

475.62

Formula

C26H41N3O5
CAS No.
Unlabeled Cas

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (210.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1025 mL 10.5126 mL 21.0252 mL
5 mM 0.4205 mL 2.1025 mL 4.2050 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1025 mL 10.5126 mL 21.0252 mL 52.5630 mL
5 mM 0.4205 mL 2.1025 mL 4.2050 mL 10.5126 mL
10 mM 0.2103 mL 1.0513 mL 2.1025 mL 5.2563 mL
15 mM 0.1402 mL 0.7008 mL 1.4017 mL 3.5042 mL
20 mM 0.1051 mL 0.5256 mL 1.0513 mL 2.6281 mL
25 mM 0.0841 mL 0.4205 mL 0.8410 mL 2.1025 mL
30 mM 0.0701 mL 0.3504 mL 0.7008 mL 1.7521 mL
40 mM 0.0526 mL 0.2628 mL 0.5256 mL 1.3141 mL
50 mM 0.0421 mL 0.2103 mL 0.4205 mL 1.0513 mL
60 mM 0.0350 mL 0.1752 mL 0.3504 mL 0.8760 mL
80 mM 0.0263 mL 0.1314 mL 0.2628 mL 0.6570 mL
100 mM 0.0210 mL 0.1051 mL 0.2103 mL 0.5256 mL
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Z-LLNle-CHO Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Z-LLNle-CHO133407-83-7Z-Leu-Leu-Nle-CHO GSIIγ-secretaseProteasomeApoptosisGamma secretaseMCF-7BT474T47DMDA-MB-231SKBR3MDA-MB-468 cellsprecursor-B ALLbreast cancerleukaemiaInhibitorinhibitorinhibit

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