2. Epigenetics TGF-beta/Smad Apoptosis
  3. PKC Apoptosis
  4. ζ-Stat

ζ-Stat (NSC37044) is a specific and atypical PKC-ζ inhibitor, with an IC50 of 5 μM. ζ-Stat can reduce melanoma cell lines proliferation and induce apoptosis, and has antitumor activity in vitro.

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ζ-Stat Chemical Structure

ζ-Stat Chemical Structure

CAS No. : 3316-02-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
 USD 396 In-stock
Solution
10 mM * 1 mL in Water USD 396 In-stock
Solid
5 mg USD 360 In-stock
10 mg USD 570 In-stock
50 mg USD 1700 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of ζ-Stat:

ζ-Stat Related Antibodies

Top Publications Citing Use of Products

View All PKC Isoform Specific Products:

View All Isoforms
PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKCι

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ζ-Stat (NSC37044) is a specific and atypical PKC-ζ inhibitor, with an IC50 of 5 μM. ζ-Stat can reduce melanoma cell lines proliferation and induce apoptosis, and has antitumor activity in vitro[1][2].

IC50 & Target[1]

aPKC-ζ

5 μM (IC50)

In Vitro

ζ-Stat (0.1-20 µM) shows only 13% inhibition on PKC-ι at 20 μM, but shows a significant inhibition on PKC-ζ as 51% at 5 μM level[1].
ζ-Stat (0.1-10 µM; 3 d) significantly decreases cell proliferation of SK-MEL-2 and MeWo upon increasing the concentrations[1].
ζ-Stat (7 or 10 µM; 24-72 h) and 5-FU in combination is able to decrease the viability of LoVo CRC cells by more than 75%[2].
ζ-Stat (5 µM; 3 d) shows a significant diminution of phosphorylated, total PKC-ζ, Bcl-2 and PARP levels, and increases Caspase-3 and cleaved-PARP levels in SK-MEL-2 and MeWo cells[1].
ζ-Stat (5 µM; 1-10 h) does not show significant cytotoxicity on MEL-F-NEO, SK-MEL-2 and MeWo cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ζ-Stat Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MEL-F-NEO, SK-MEL-2 and MeWo cells
Concentration: 0.1, 0.5, 1, 2.5, 5, 7.5, 10 µM
Incubation Time: 3 days
Result: Decreased proliferation by 47.7% for 5 µM in SK-MEL-2 cells and by 50.6% for 5 µM in MeWo cells.
Showed significant inhibitions on MEL-F-NEO cells 19.3% (P ≤ 0.05) at 10 μM.

Western Blot Analysis[1]

Cell Line: SK-MEL-2 and MeWo cells
Concentration: 5 µM
Incubation Time: 3 days
Result: Decreased phosphorylated and total PKC-ζ levels.
Molecular Weight

384.36

Formula

C10H8O10S3
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=S(C1=C2C(O)=CC(S(=O)(O)=O)=CC2=CC(S(=O)(O)=O)=C1)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (162.61 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6017 mL 13.0086 mL 26.0173 mL
5 mM 0.5203 mL 2.6017 mL 5.2035 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (260.17 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.6017 mL 13.0086 mL 26.0173 mL 65.0432 mL
5 mM 0.5203 mL 2.6017 mL 5.2035 mL 13.0086 mL
10 mM 0.2602 mL 1.3009 mL 2.6017 mL 6.5043 mL
15 mM 0.1734 mL 0.8672 mL 1.7345 mL 4.3362 mL
20 mM 0.1301 mL 0.6504 mL 1.3009 mL 3.2522 mL
25 mM 0.1041 mL 0.5203 mL 1.0407 mL 2.6017 mL
30 mM 0.0867 mL 0.4336 mL 0.8672 mL 2.1681 mL
40 mM 0.0650 mL 0.3252 mL 0.6504 mL 1.6261 mL
50 mM 0.0520 mL 0.2602 mL 0.5203 mL 1.3009 mL
60 mM 0.0434 mL 0.2168 mL 0.4336 mL 1.0841 mL
80 mM 0.0325 mL 0.1626 mL 0.3252 mL 0.8130 mL
100 mM 0.0260 mL 0.1301 mL 0.2602 mL 0.6504 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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ζ-Stat Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ζ-Stat3316-02-7NSC37044NSC 37044NSC-37044PKCApoptosisProtein kinase CatypicalPKC-ζmelanomaapoptosisantitumorproteinkinaseCInhibitorinhibitorinhibit

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