2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Phosphatase Reactive Oxygen Species Endogenous Metabolite
  4. L-Ascorbic acid 2-phosphate trisodium

L-Ascorbic acid 2-phosphate trisodium  (Synonyms: 2-Phospho-L-ascorbic acid trisodium)

Cat. No.: HY-107837 Purity: 99.45%
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L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) is a long-acting vitamin C derivative that can stimulate collagen formation and expression. L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation.

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L-Ascorbic acid 2-phosphate trisodium Chemical Structure

L-Ascorbic acid 2-phosphate trisodium Chemical Structure

CAS No. : 66170-10-3

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Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

Other Forms of L-Ascorbic acid 2-phosphate trisodium:

L-Ascorbic acid 2-phosphate trisodium Related Antibodies

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1 Publications Citing Use of MCE L-Ascorbic acid 2-phosphate trisodium

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Description

L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) is a long-acting vitamin C derivative that can stimulate collagen formation and expression[1]. L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation[2][3].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) trisodium significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect [1].
L-Ascorbic acid 2-phosphate (50 µM-250 µM) trisodium is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 µM AsA2-P and 10 nM dexamethasone (Dex), and 250 µM AsA2-P and 5 nM Dex[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Ascorbic acid 2-phosphate trisodium Related Antibodies
Molecular Weight

322.05

Formula

C6H6Na3O9P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H](CO)[C@@H](O1)C(O[Na])=C(OP(O[Na])(O[Na])=O)C1=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 150 mg/mL (465.77 mM; Need ultrasonic)

DMSO : 6 mg/mL (18.63 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1051 mL 15.5255 mL 31.0511 mL
5 mM 0.6210 mL 3.1051 mL 6.2102 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 120 mg/mL (372.61 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.45%

COA (252 KB) RP-HPLC (208 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.1051 mL 15.5255 mL 31.0511 mL 77.6277 mL
5 mM 0.6210 mL 3.1051 mL 6.2102 mL 15.5255 mL
10 mM 0.3105 mL 1.5526 mL 3.1051 mL 7.7628 mL
15 mM 0.2070 mL 1.0350 mL 2.0701 mL 5.1752 mL
H2O 20 mM 0.1553 mL 0.7763 mL 1.5526 mL 3.8814 mL
25 mM 0.1242 mL 0.6210 mL 1.2420 mL 3.1051 mL
30 mM 0.1035 mL 0.5175 mL 1.0350 mL 2.5876 mL
40 mM 0.0776 mL 0.3881 mL 0.7763 mL 1.9407 mL
50 mM 0.0621 mL 0.3105 mL 0.6210 mL 1.5526 mL
60 mM 0.0518 mL 0.2588 mL 0.5175 mL 1.2938 mL
80 mM 0.0388 mL 0.1941 mL 0.3881 mL 0.9703 mL
100 mM 0.0311 mL 0.1553 mL 0.3105 mL 0.7763 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Ascorbic acid 2-phosphate trisodium Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Ascorbic acid 2-phosphate trisodium66170-10-32-Phospho-L-ascorbic acid trisodiumPhosphataseReactive Oxygen SpeciesEndogenous MetabolitehASCosteogenicdifferentiationmediumsupplementrunx2AvitaminCderivativeInhibitorinhibitorinhibit

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