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2-Methylnicotinic acid imidazolide 

Cat. No.: HY-123992
Handling Instructions

2-Methylnicotinic acid imidazolide, a RNA-SHAPE electrophile, is a general cell-permeable probe of RNA structure. 2-Methylnicotinic acid imidazolide can probe RNA structure in vivo and in live cells with high accuracy and single-nucleotide resolution.

For research use only. We do not sell to patients.

2-Methylnicotinic acid imidazolide Chemical Structure

2-Methylnicotinic acid imidazolide Chemical Structure

CAS No. : 1055970-47-2

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Description

2-Methylnicotinic acid imidazolide, a RNA-SHAPE electrophile, is a general cell-permeable probe of RNA structure. 2-Methylnicotinic acid imidazolide can probe RNA structure in vivo and in live cells with high accuracy and single-nucleotide resolution[1].

In Vitro

Application of 100 mM 2-Methylnicotinic acid imidazolide (NAI) to murine embryonic stem cells (ESCs) resulted in 5S rRNA modification in as little as one minute with suitable signal-to-noise ratio. This signal begins to plateau by 15 minutes. 2-Methylnicotinic acid imidazolide also modifies 5S rRNA in cultured human cancer cells, Drosophila S2 cells, yeast, and E. coli. 2-Methylnicotinic acid imidazolide can read out alterations in the RNA tertiary structure as a result of sampling critical mature ribosome conformations.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

187.20

Formula

C₁₀H₉N₃O

CAS No.
SMILES

O=C(N1C=CN=C1)C2=CC=CN=C2C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (267.09 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.3419 mL 26.7094 mL 53.4188 mL
5 mM 1.0684 mL 5.3419 mL 10.6838 mL
10 mM 0.5342 mL 2.6709 mL 5.3419 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (13.35 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (13.35 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (13.35 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

2-Methylnicotinic acid imidazolideOtherselectrophileRNAstructureSHAPElivecellscell-permeableInhibitorinhibitorinhibit

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Product Name:
2-Methylnicotinic acid imidazolide
Cat. No.:
HY-123992
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