5-LOX-IN-4

Cat. No.: HY-U00308 Purity: 98.07%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-LOX-IN-4 is a 5-Lipoxygenase inhibitor extracted from patent EP 331232 A2, table 4, compound example 4.10.

For research use only. We do not sell to patients.

5-LOX-IN-4 Chemical Structure

CAS No. : 125235-15-6

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 715	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 715	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2950	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4500	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

5-LOX-IN-4 is a 5-Lipoxygenase inhibitor extracted from patent EP 331232 A2, table 4, compound example 4.10.

IC₅₀ & Target

5-Lipoxygenase

Molecular Weight

424.56

Formula

C₂₃H₂₈N₄O₂S

CAS No.

125235-15-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(C(C)(C)N1CC)N(C2=CC=C(N3CCN(C4=CC=C(O)C=C4)CC3)C=C2)C1=S

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (588.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3554 mL	11.7769 mL	23.5538 mL
5 mM	0.4711 mL	2.3554 mL	4.7108 mL
10 mM	0.2355 mL	1.1777 mL	2.3554 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.07%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (248 KB) HNMR (218 KB) LCMS (112 KB)

Handling Instructions (2659 KB)

References

[1]. Wauwe V, et al. 5-Lipoxygenase-inhibiting 4-(4-phenyl-1-piperazinyl)phenols and their preparation and pharmaceutical compositions. EP0331232A2

Kinase Assay ^[1]	The enzyme activity is assayed at 37°C in a reaction mixture (total volume of 0.4 mL) containing 50 mM sodium phosphate buffer (pH7.4), 2mM ATP, 2mM CaC1₂, 2mM glutathion, 5-Lipoxygenase-IN-1 (10^-5 to 10^-8 M) and the enzyme (60 mg protein). For inhibition studies, concentration response curves and IC₅₀-values are obtained by determining the percentage of inhibition of lipoxygenase products formation in the presence of 5-Lipoxygenase-IN-1 compared with the uninhibited control^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] Unfasted male Swiss mice weighing 24-26 g are used in the experiments. The mice are treated orally with 5-Lipoxygenase-IN-1 dissolved in a volume of 150 mL of either polyethyleneglycol (PEG 200) of hydroxypropyl cyclodextrine at doses varying between 1.25 and 40 mg per kg bodyweight. In control experiments the mice are administered an indentical amount of solvent alone. One hour after treatment there is injected intravenously an isotonic saline solution containing 60 mg/mL Dextran T5000 and 13 mg/mL pontamine sky-blue dye in a volume of 0.1 mL per 10 bodyweight. One hour and forty-five minutes later the animals are sacrificed by ether and their ears are removed. Extraction and quantification of the extravasated dye are performed^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Wauwe V, et al. 5-Lipoxygenase-inhibiting 4-(4-phenyl-1-piperazinyl)phenols and their preparation and pharmaceutical compositions. EP0331232A2

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3554 mL	11.7769 mL	23.5538 mL	58.8845 mL
	5 mM	0.4711 mL	2.3554 mL	4.7108 mL	11.7769 mL
	10 mM	0.2355 mL	1.1777 mL	2.3554 mL	5.8884 mL
	15 mM	0.1570 mL	0.7851 mL	1.5703 mL	3.9256 mL
	20 mM	0.1178 mL	0.5888 mL	1.1777 mL	2.9442 mL
	25 mM	0.0942 mL	0.4711 mL	0.9422 mL	2.3554 mL
	30 mM	0.0785 mL	0.3926 mL	0.7851 mL	1.9628 mL
	40 mM	0.0589 mL	0.2944 mL	0.5888 mL	1.4721 mL
	50 mM	0.0471 mL	0.2355 mL	0.4711 mL	1.1777 mL
	60 mM	0.0393 mL	0.1963 mL	0.3926 mL	0.9814 mL
	80 mM	0.0294 mL	0.1472 mL	0.2944 mL	0.7361 mL
	100 mM	0.0236 mL	0.1178 mL	0.2355 mL	0.5888 mL

5-LOX-IN-4 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-LOX-IN-4125235-15-6LipoxygenaseLOXInflammatorycancerstrokeAlzheimer's diseaseInhibitorinhibitorinhibit

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