1. Metabolic Enzyme/Protease
  2. Lipoxygenase
  3. 5-Lipoxygenase-In-1

5-Lipoxygenase-In-1 

Cat. No.: HY-U00308
Handling Instructions

5-Lipoxygenase-In-1 is a 5-Lipoxygenase inhibitor extracted from patent EP 331232 A2, table 4, compound example 4.10.

For research use only. We do not sell to patients.

5-Lipoxygenase-In-1 Chemical Structure

5-Lipoxygenase-In-1 Chemical Structure

CAS No. : 125235-15-6

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 935 In-stock
Estimated Time of Arrival: December 31
5 mg USD 850 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1300 In-stock
Estimated Time of Arrival: December 31
25 mg USD 2450 In-stock
Estimated Time of Arrival: December 31
50 mg USD 3800 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

View All Lipoxygenase Isoform Specific Products:

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

5-Lipoxygenase-In-1 is a 5-Lipoxygenase inhibitor extracted from patent EP 331232 A2, table 4, compound example 4.10.

IC50 & Target

5-Lipoxygenase

 

Molecular Weight

424.56

Formula

C₂₃H₂₈N₄O₂S

CAS No.

125235-15-6

SMILES

O=C(C(C)(C)N1CC)N(C2=CC=C(N3CCN(C4=CC=C(O)C=C4)CC3)C=C2)C1=S

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (588.84 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3554 mL 11.7769 mL 23.5538 mL
5 mM 0.4711 mL 2.3554 mL 4.7108 mL
10 mM 0.2355 mL 1.1777 mL 2.3554 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[1]

The enzyme activity is assayed at 37°C in a reaction mixture (total volume of 0.4 mL) containing 50 mM sodium phosphate buffer (pH7.4), 2mM ATP, 2mM CaC12, 2mM glutathion, 5-Lipoxygenase-In-1 (10-5 to 10-8 M) and the enzyme (60 mg protein). For inhibition studies, concentration response curves and IC50-values are obtained by determining the percentage of inhibition of lipoxygenase products formation in the presence of 5-Lipoxygenase-In-1 compared with the uninhibited control[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]

Unfasted male Swiss mice weighing 24-26 g are used in the experiments. The mice are treated orally with 5-Lipoxygenase-In-1 dissolved in a volume of 150 mL of either polyethyleneglycol (PEG 200) of hydroxypropyl cyclodextrine at doses varying between 1.25 and 40 mg per kg bodyweight. In control experiments the mice are administered an indentical amount of solvent alone. One hour after treatment there is injected intravenously an isotonic saline solution containing 60 mg/mL Dextran T5000 and 13 mg/mL pontamine sky-blue dye in a volume of 0.1 mL per 10 bodyweight. One hour and forty-five minutes later the animals are sacrificed by ether and their ears are removed. Extraction and quantification of the extravasated dye are performed[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

5-Lipoxygenase-In-1LipoxygenaseLOXInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
5-Lipoxygenase-In-1
Cat. No.:
HY-U00308
Quantity:
MCE Japan Authorized Agent: