1. Cell Cycle/DNA Damage
    Autophagy
    Apoptosis
  2. Eukaryotic Initiation Factor (eIF)
    Autophagy
    Apoptosis
  3. 4EGI-1

4EGI-1 

製品番号: HY-19831 純度: 98.83%
取扱説明書

4EGI-1 is an inhibitor of eIF4E/eIF4G interaction, with a Kd of 25 μM against eIF4E binding.

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4EGI-1 構造式

4EGI-1 構造式

CAS 番号 : 315706-13-9

容量 価格(税別) 在庫状況 数量
無料サンプル (0.5-1 mg)   今すぐ申し込む  
10 mM * 1 mL in DMSO USD 119 在庫あり
Estimated Time of Arrival: December 31
5 mg USD 108 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 144 在庫あり
Estimated Time of Arrival: December 31
25 mg USD 264 在庫あり
Estimated Time of Arrival: December 31
50 mg USD 420 在庫あり
Estimated Time of Arrival: December 31
100 mg USD 672 在庫あり
Estimated Time of Arrival: December 31
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

カスタマーレビュー

Based on 6 publication(s) in Google Scholar

Other Forms of 4EGI-1:

Top Publications Citing Use of Products

    4EGI-1 purchased from MCE. Usage Cited in: Nat Commun. 2017 Jun 8;8:15617.

    Immunoblot analysis of KRAS protein levels in parental (P) and resistant derivatives (R1 and R2) following 4 h treatment with the corresponding inhibitors Rapamycin, AZD2014, MLN0128, BEZ235 and 4EGI-1. Images are cropped for clarity from the same exposure of the same membrane.

    4EGI-1 purchased from MCE. Usage Cited in: Mol Cancer Ther. 2018 Dec;17(12):2676-2688.

    Immunoblot assessing protein synthesis, using puromycin labeling, and CHK1 levels of cell lines treated with the protein translation inhibitor 4EGI-1.
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    製品説明

    4EGI-1 is an inhibitor of eIF4E/eIF4G interaction, with a Kd of 25 μM against eIF4E binding.

    IC50 & Target

    Kd: 25 μM (eIF4E/eIF4G)[1]

    体外実験

    4EGI-1 is an inhibitor of eIF4E/eIF4G interaction, with a Kd of 25 μM against eIF4E binding. 4EGI-1 disrupts the eIF4F complex and inhibits expression of oncogenic proteins in mammalian cells. 4EGI-1 (0-40 μM) also exhibits proapoptotic activity and inhibits the growth of multiple cancer cell lines[1]. 4EGI-1 is cytotoxic to breast cancer cells, such as SKBR-3, MCF-7 and MDA-MB-231 cells, with the IC50 of appr 30 μM, and to the non-CSCs (Cancer stem cells), the IC50 is about 22 μM. 4EGI-1 enhances breast CSC differentiation (40 μM), and suppresses breast CSC induced HUVEC tube-like structure formation (8 μM). Moreover, 4EGI-1 selectively inhibits translation that persists in CSC maintenance and dissemination[2]. 4EGI-1 (50 µM) impairs the formation of eIF4F complex in U87 cells. 4EGI-1 (10, 50 and 100 µM) inhibits cell proliferation via inducing apoptosis in U87 cells, and the apoptosis is via Bax activation. 4EGI-1 causes mitochondrial dysfunction, and induces ER stress via GRP-78 activation, in U87 cells[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内実験

    4EGI-1 (75 mg/kg, i.p.) inhibits breast cancer stem cells (CSC) tumor growth and tumorangiogenesis in vivo[2]. 4EGI-1 (75 mg/kg, i.p.) shows inhibitory effect on the tumor volume and weight in mice bearing U87 cells[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    451.28

    分子式

    C₁₈H₁₂Cl₂N₄O₄S

    CAS 番号

    315706-13-9

    SMILES

    O=C(O)/C(CC1=CC=CC=C1[N+]([O-])=O)=N/NC2=NC(C3=CC=C(Cl)C(Cl)=C3)=CS2

    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 35 mg/mL (77.56 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2159 mL 11.0796 mL 22.1592 mL
    5 mM 0.4432 mL 2.2159 mL 4.4318 mL
    10 mM 0.2216 mL 1.1080 mL 2.2159 mL
    *Please refer to the solubility information to select the appropriate solvent.
    体内:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (5.54 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are provided by MCE.
    参考文献
    細胞実験
    [2]

    1 × 104 breast CSCs HMLER (CD44high/CD24low)FA cells and other indicated breast cancer cells are treated with DMSO, or [E]-4EGI-1 or [Z]-4EGI-1 at series of concentrations for 24 hours. The cells are performed cell viability assays with cell viability assay kit. Three independent experiments are performed. Average IC50 results are shown (mean ± SD, t-test, two-tailed)[2].

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    動物実験
    [2]

    Mice[2]
    In the tumor xenografted assay, 1×105 breast cancer stem cells (CSCs) are mixed with 100 μL Matrigel/DMEM mixture (Matrigel: DMEM = 1:2). Breast CSCs/Matrigel/DMEM mixtures are injected into NOD/SCID female mice mammary glands by subcutaneous injection. After the tumor formation (about 75 mm3 in volume, 5 mice/group), DMSO, or 75 mg/kg [E]-4EGI-1, or 75 mg/kg [Z]-4EGI-1 is injected into the mice by intraperitoneal injection daily for 30 days. Tumor volumes are measured every three days. At the 30th day, mice are sacrificed and tumors are excised. Tumors weights are measured. Tumor tissue samples are used for immunohistostaining, Western blot and immunoprecipitation analyses[2].

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    参考文献

    純度: 98.83%

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    Keywords:

    4EGI-1Eukaryotic Initiation Factor (eIF)AutophagyApoptosisInhibitorinhibitorinhibit

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    製品名:
    4EGI-1
    製品番号:
    HY-19831
    数量:
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