1. Metabolic Enzyme/Protease
  2. Phospholipase
  3. 7-Hydroxycoumarinyl-γ-linolenate

7-Hydroxycoumarinyl-γ-linolenate 

Cat. No.: HY-D1647
Handling Instructions

7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A2 (cPLA2) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA2.

For research use only. We do not sell to patients.

7-Hydroxycoumarinyl-γ-linolenate Chemical Structure

7-Hydroxycoumarinyl-γ-linolenate Chemical Structure

CAS No. : 161180-12-7

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Description

7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A2 (cPLA2) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA2[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
cPLA2α activity assay[1][2]:
1. Sonicate 200 μL of 1,2-O-tetradecyl-sn-glycero-3-phosphocholine (DTPC)/Triton X-100 high-affinity mixed micelle solution containing 960 μM Triton X-100 (HY-Y1883A), 60 μM DTPC, 50 μM 7-hydroxycoumarinyl-γ-linolenate, 50 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (pH = 7.2), 0.3 mM EDTA (HY-Y0682), 1 mM CaCl2 and 300 mM KCl.
2. Monitoring the hydrolysis of 7-hydroxycoumarinyl-γ-linolenate continuously with excitation at 360 nm and emission at 460 nm.
3. The formation of 7-hydroxycoumarin was monitored by fluorospectrometer.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

422.56

Formula

C27H34O4

CAS No.
SMILES

CCCCC/C=C\C/C=C\C/C=C\CCCCC(OC1=CC=C(C(O2)=C1)C=CC2=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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Product Name:
7-Hydroxycoumarinyl-γ-linolenate
Cat. No.:
HY-D1647
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