1. Metabolic Enzyme/Protease
  2. FXR
    Endogenous Metabolite
  3. Androsterone

Androsterone (Synonyms: 5α-Androstan-3α-ol-17-one)

Cat. No.: HY-N0933 Purity: >98.0%
Handling Instructions

Androsterone is a metabolic product of testosterone and can activate Farnesoid X Receptor (FXR).

For research use only. We do not sell to patients.

Androsterone Chemical Structure

Androsterone Chemical Structure

CAS No. : 53-41-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
250 mg USD 80 In-stock
Estimated Time of Arrival: December 31
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Description

Androsterone is a metabolic product of testosterone and can activate Farnesoid X Receptor (FXR).

IC50 & Target

FXR[1]

In Vitro

Androsterone activates both the mFXR-LBD and the hFXR-LBD, with Androsterone activating the mFXR-LBD more strongly than the hFXR-LBD. Furthermore, cotransfection studies with gal4-hFXR-LBD and SRC-1/VP16 expression plasmids demonstrate that Androsterone potentiates the interaction of SRC-1 with the hFXR-LBD. Several amino acid changes including H294S, S332V, R351H, and Y361F significantly reduce Androsterone activation[1]. Androsterone (5α, 3α-A) (10 to 100 μM) also inhibits epileptiform discharges in a concentration-dependent fashion in the in vitro slice model[2].

In Vivo

Androsterone treatment results in a significant induction of small heterodimer partner (SHP), suggesting Androsterone may activate endogenous FXR[1]. Intraperitoneal injection of Androsterone (5α, 3α-A) protects mice in a dose-dependent fashion from seizures in the following models (ED50, dose in mg/kg protecting 50% of animals): 6 Hz electrical stimulation (29.1), pentylenetetrazol (43.5), pilocarpine (105), 4-AP (215), and maximal electroshock (224)[2].

Molecular Weight

290.44

Formula

C₁₉H₃₀O₂

CAS No.

53-41-8

SMILES
Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (172.15 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4431 mL 17.2153 mL 34.4305 mL
5 mM 0.6886 mL 3.4431 mL 6.8861 mL
10 mM 0.3443 mL 1.7215 mL 3.4431 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 3.25 mg/mL (11.19 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

AML-12 cells are used and cultured in a 1:1 mix of DMEM/Ham’s F12 medium supplemented with 10% fetal bovine serum, 1% insulin-transferrin-selenium mix, 40 ng/mL dexamethasone, 100 U/mL penicillin, and 100 μg/mL streptomycin. For gene regulation studies, AML-12 cells are plated in growth medium at 4×105 cells per well in six-well plates. The following day, treatments including Androsterone or dimethylsulfoxide (DMSO) vehicle are added to the medium. At various times after treatment addition, total RNA is prepared. mRNA levels for individual genes are determined by real-time PCR[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Castrated male mice 8 to10 wk of age are fed a casein diet for 2 wk before the start of the study. The mice receive daily sc injections of 0.1 mL 90% corn oil/10% ethanol vehicle or 10 mg/mL Androsterone in vehicle. Animals are killed 2 h after the fifth daily treatment, approximately 4 h after commencement of the light cycle. Total RNA is prepared, and mRNA levels for specific genes are determined by real-time PCR[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Androsterone
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HY-N0933
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