1. Cell Cycle/DNA Damage
    Stem Cell/Wnt
  2. Casein Kinase
  3. Casein Kinase II Inhibitor IV Hydrochloride

Casein Kinase II Inhibitor IV Hydrochloride 

Cat. No.: HY-111378A
Handling Instructions

Casein Kinase II Inhibitor IV Hydrochloride is a small-molecule inducer of epidermal keratinocyte differentiation.

For research use only. We do not sell to patients.

Casein Kinase II Inhibitor IV Hydrochloride Chemical Structure

Casein Kinase II Inhibitor IV Hydrochloride Chemical Structure

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50 mg USD 1850 Get quote

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Description

Casein Kinase II Inhibitor IV Hydrochloride is a small-molecule inducer of epidermal keratinocyte differentiation.

IC50 & Target

Target: Casein Kinase[1]

In Vitro

Treatment of human epidermal keratinocytes (NHEKs) with Casein Kinase II Inhibitor IV leads to an increase in the early differentiation markers keratins 1 and 10 at 48 h. Increased levels of IVL and TGM are observed in cells treated with Casein Kinase II Inhibitor IV at 72 h and persisted at 96 h. In addition, treated with Casein Kinase II Inhibitor IV expresses loricrin, a terminal differentiation marker, at later time points. Similar results are observed by messenger RNA (mRNA) expression analysis of NHEKs treated with Casein Kinase II Inhibitor IV. At early time points (12 and 24 h), treatment with Casein Kinase II Inhibitor IV leads to the upregulation of keratinocyte early differentiation marker genes, including keratin 1 (5.4-fold) and keratin 10 (5.4-fold). Terminal differentiation marker genes, including IVL (1.8-fold), TGM 1 (4.8-fold), loricrin (3.3-fold), and filaggrin (5.6-fold), are upregulated at late time points (36 and 48 h). These results are consistent with the ability of Casein Kinase II Inhibitor IV to induce differentiation of epidermal progenitor cells into terminally differentiated keratinocytes[1].

Molecular Weight

465.93

Formula

C₂₄H₂₄ClN₅O₃

SMILES

N#CCCC1=CC=CC(N2C=CC3=CN=C(NC4=CC(OC)=C(OC)C(OC)=C4)N=C32)=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Kinase Assay
[1]

For reporter gene assays with transiently transfected cells, the cells are typically transfected in 150 mm-diam dishes when 30-40% confluent. A reporter plasmid, pGL3/3.7 kbp-IVLLuc plasmid, is transfected into the human epidermal keratinocytes (NHEKs). After 24 h, the transfected cells are plated into 96-well assay plates and treated with Casein Kinase II Inhibitor IV to a final concentration of 5 μM. After incubation for 2 d, reporter gene activity is measured using the Bright-Glo luciferase assay system[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Product Name:
Casein Kinase II Inhibitor IV Hydrochloride
Cat. No.:
HY-111378A
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