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Coelenterazine 

製品番号: HY-18743 純度: 99.01%
取扱説明書

Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite .

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Coelenterazine 構造式

Coelenterazine 構造式

CAS 番号 : 55779-48-1

容量 価格(税別) 在庫状況 数量
500 μg USD 60 在庫あり
Estimated Time of Arrival: December 31
5 mg USD 350 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 610 在庫あり
Estimated Time of Arrival: December 31
25 mg USD 1350 在庫あり
Estimated Time of Arrival: December 31
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

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製品説明

Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite[1] [2][3].

体外実験

HCT-8 control cells, transiently expressing Renilla luciferase (RLuc), showed low bioluminescence due to P-glycoprotein–mediated efflux transport of coelenterazine. By comparison, transiently expressing RLuc HCT-8 cells, wherein P-glycoprotein was down-regulated with shRNAi, showed high bioluminescence[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

The in vivo growth potential of HCC1806-RR was monitored by injecting animals with coelenterazine (2 mg/kg) i.v. and exposing them to a charged-coupled device (CCD) camera 5 minutes later. Rluc activity was detected as light emitted from the tumor cells and acquired as a pseudo-color image superimposed over a black and white photograph of the animal. All mice demonstrated very high Rluc activity at the primary site with the majority of mice simultaneously showing metastases to inguinal ILNs[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

423.46

分子式

C₂₆H₂₁N₃O₃

CAS 番号

55779-48-1

SMILES

O=C1C(CC2=CC=C(O)C=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=C(O)C=C5)=CN31

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

溶剤 & 溶解度
体外: 

Ethanol : 2 mg/mL (4.72 mM; Need ultrasonic; DMSO can inactivate Coelenterazine's activity)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3615 mL 11.8075 mL 23.6150 mL
5 mM --- --- ---
10 mM --- --- ---
*Please refer to the solubility information to select the appropriate solvent.
体内:
  • 1.

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.2 mg/mL (0.47 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.2 mg/mL (0.47 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% EtOH    90% Saline

    Solubility: ≥ 0.2 mg/mL (0.47 mM); Clear solution

*All of the co-solvents are provided by MCE.
参考文献
細胞実験
[1]

Cells are plated at a density of 5×104 cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2 and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells[1].

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

動物実験
[1]

Mice are anesthetized with metofane or isoflurane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. Anesthesia is maintained during imaging by nose cone delivery of 2.5% isoflurane. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed[1].

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

参考文献

純度: 99.01%

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This equation is commonly abbreviated as: C1V1 = C2V2

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Keywords:

CoelenterazineOthersInhibitorinhibitorinhibit

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製品名:
Coelenterazine
製品番号:
HY-18743
数量:
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