1. GPCR/G Protein
    Metabolic Enzyme/Protease
    Autophagy
  2. Adenylate Cyclase
    FXR
    Autophagy
  3. Forskolin

Forskolin (Synonyms: Coleonol; Colforsin)

製品番号: HY-15371 純度: 99.82%
取扱説明書

Forskolin (Coleonol) is a potent adenylate cyclase activator with an IC50 of 41 nM and an EC50 of 0.5 μM for type I adenylyl cyclase. Forskolin is also an inducer of intracellular cAMP formation. Forskolin induces differentiation of various cell types and activates pregnane X receptor (PXR) and FXR. Forskolin exerts a inotropic effect on the heart, and has platelet antiaggregatory and antihypertensive actions. Forskolin also induces autophagy.

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Forskolin 構造式

Forskolin 構造式

CAS 番号 : 66575-29-9

容量 価格(税別) 在庫状況 数量
10 mM * 1 mL in DMSO USD 72 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 65 在庫あり
Estimated Time of Arrival: December 31
50 mg USD 130 在庫あり
Estimated Time of Arrival: December 31
100 mg USD 180 在庫あり
Estimated Time of Arrival: December 31
200 mg USD 234 在庫あり
Estimated Time of Arrival: December 31
500 mg   お問い合わせ  
1 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

カスタマーレビュー

Based on 29 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Forskolin purchased from MCE. Usage Cited in: J Lipid Res. 2018 Feb;59(2):330-338.

    Forskolin (FSK) -stimulated dephsphorylation of HDAC5 is also inhibited by Thapsigargin (THA) treatment in primary hepatocytes.

    Forskolin purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Jan;120(1):321-331.

    The combination of Fsk and IBMX (Fsk-IBMX) inhibits the expression of cAMP related protein. The results of Western blot in glioma stem cells (GSCs).

    Forskolin purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Jan;120(1):321-331.

    U0126 enhances the negative effect of Fsk-IBMX on the development of glioma stem cells (GSCs).
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    製品説明

    Forskolin (Coleonol) is a potent adenylate cyclase activator with an IC50 of 41 nM and an EC50 of 0.5 μM for type I adenylyl cyclase[1]. Forskolin is also an inducer of intracellular cAMP formation[2]. Forskolin induces differentiation of various cell types and activates pregnane X receptor (PXR) and FXR[3]. Forskolin exerts a inotropic effect on the heart, and has platelet antiaggregatory and antihypertensive actions. Forskolin also induces autophagy[4][5].

    IC50 & Target

    IC50: 41 nM (Adenylyl cyclase)[1]
    EC50: 0.5 μM (Adenylyl cyclase)[1]

    体外実験

    Forskolin (Coleonol) is also a potent exosome biogenesis and/or secretion activator in prostate cancer (PC) cells[8].
    Forskolin (Fsk) is a naturally occurring diterpene isolated from Coleus forskholii, directly activates adenylyl cyclase (AC) through its catalytic subunit to increase intracellular levels of cyclic adenosine monophosphate (cAMP)[1].
    Forskolin (Fsk) affects the proliferation of the human T-cell lines such as Kit 225 and MT-2. Forskolin treatment inhibits the proliferation of both Kit 225 and MT-2 cells in a dose-dependent manner with an IC50 equal to ~5 μM Fsk. Forskolin treatment (10-100 μM) increases cAMPi levels ~5- to 20-fold above basal levels, which reache maximum levels between 50-100 μM Forskolin[6].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内実験

    The Forskolin (Coleonol)-treated Mrp4-/- mice shows an increased number of Ki67-positive and cleaved caspase 3-positive ECs, a significant decrease in the amount of pericyte coverage, and a reduced number of empty sleeves. In pups exposed to hyperoxia (75% oxygen) from P7 to P12, the Mrp4-/- mice shows a significant increase in the unvascularized retinal area[2].
    The average blood glucose in the healthy rat group is 102.12±1.94 mg/dL, 101.25±3.56 for control group and 103±2.08 in forskolin group. The data shows that glucose levels at the end of the study are lower in forskolin group, with a significant difference according to the statistical tests applied (p=0.03)[7].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    臨床実験
    分子量

    410.50

    分子式

    C₂₂H₃₄O₇

    CAS 番号

    66575-29-9

    SMILES
    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (243.61 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4361 mL 12.1803 mL 24.3605 mL
    5 mM 0.4872 mL 2.4361 mL 4.8721 mL
    10 mM 0.2436 mL 1.2180 mL 2.4361 mL
    *Please refer to the solubility information to select the appropriate solvent.
    体内:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    参考文献
    細胞実験
    [2]

    Quiescent Kit 225 or MT-2 cells are seeded into 96-well plates at 5×104 cells per well. Cells are then pretreated for 1 h with 1% DMSO (vehicle) or Forskolin at 1, 5, 10, 25, 50, and 100 μMconcentrations. The cells are stimulated with IL-2 and cultured for an additional 20 h at 37°C. Control cells are treated with 1% DMSO for 20 h. During the final 4 h of incubation, the cells are pulsed with [3H]thymidine at a concentration of 0.5 μCi/200 μL. Cells are harvested onto fiberglass filters and analyzed using liquid scintillation counting[2].

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    動物実験
    [3][4]

    Mice[3]
    C57BL/6J mice are used. Mrp4-knockout mice, which are established and repeatedly backcrossed to the C57BL/6J mice. Forskolin is injected intraperitoneally into neonatal mice at postnatal days 4 (P4) and 5 (P5). Mice injected with DMSO serve as the controls. The treated mice are euthanized at P6, and their retinas are isolated for whole-mount immunohistochemistry (IHC). The effect of different concentrations of Forskolin on the survival rate and retinal vasculature is first tested, and the optimal concentration is determined, 1.0 μg/50 μL (0.3 mg/kg) at P4 and 1.5 μg/50 μL (0.5 mg/kg) at P5, used to compare the retinal vascular phenotypes between WT mice and Mrp4-deficient mice.
    Rats[4]
    Male Wistar rats, aged 10-14 weeks old, with a mean weight of 300 g±50 g, are divided into four groups; 19 are experimentally induced to develop diabetes, and 8 are maintained in a healthy condition. Both diabetic and healthy rats receive no Forskolin (control), or 6 mg/kg per day of Forskolin, administered orally for 8 weeks. Blood glucose levels are determined in each group before and after Forskolin treatment. The diabetic rats are tested two weeks after confirming the presence of diabetes (three weeks after the induction) and after eight weeks of the designated treatment.

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    参考文献

    純度: 99.82%

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    Keywords:

    ForskolinColeonol ColforsinAdenylate CyclaseFXRAutophagyAdenylyl cyclaseNR1H4cAMPpregnaneX receptorPXRnotropicantiaggregatoryantihypertensiveexosomeprostatecancerInhibitorinhibitorinhibit

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    製品名:
    Forskolin
    製品番号:
    HY-15371
    数量:
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