Forskolin (Synonyms: Coleonol; Colforsin; HL 362)

製品番号: HY-15371 純度: 99.78%: FAQs
Data Sheet SDS COA 取扱説明書

Forskolin (Coleonol) is a potent adenylate cyclase activator with an IC₅₀ of 41 nM and an EC₅₀ of 0.5 μM for type I adenylyl cyclase. Forskolin is also an inducer of intracellular cAMP formation. Forskolin induces differentiation of various cell types and activates pregnane X receptor (PXR) and FXR. Forskolin exerts a inotropic effect on the heart, and has platelet antiaggregatory and antihypertensive actions. Forskolin also induces autophagy.

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Forskolin 構造式

CAS 番号 : 66575-29-9

容量	価格（税別）	在庫状況	数量

無料サンプル (0.1 - 0.5 mg)		今すぐ申し込む
Solution
10 mM * 1 mL in DMSO	USD 72	在庫あり	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 72	在庫あり	Estimated Time of Arrival: December 31
Solid
10 mg	USD 65	在庫あり	Estimated Time of Arrival: December 31
50 mg	USD 130	在庫あり	Estimated Time of Arrival: December 31
100 mg	USD 180	在庫あり	Estimated Time of Arrival: December 31
200 mg	USD 234	在庫あり	Estimated Time of Arrival: December 31
500 mg		お問い合わせ
1 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

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Based on 65 publication(s) in Google Scholar

顧客検証

: Forskolin purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Jan;120(1):321-331. [Abstract]; U0126 enhances the negative effect of Fsk-IBMX on the development of glioma stem cells (GSCs).

: Forskolin purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Jan;120(1):321-331. [Abstract]; The combination of Fsk and IBMX (Fsk-IBMX) inhibits the expression of cAMP related protein. The results of Western blot in glioma stem cells (GSCs).

: Forskolin purchased from MCE. Usage Cited in: J Lipid Res. 2018 Feb;59(2):330-338. [Abstract]; Forskolin (FSK) -stimulated dephsphorylation of HDAC5 is also inhibited by Thapsigargin (THA) treatment in primary hepatocytes.

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製品説明

Forskolin (Coleonol) is a potent adenylate cyclase activator with an IC₅₀ of 41 nM and an EC₅₀ of 0.5 μM for type I adenylyl cyclase^[1]. Forskolin is also an inducer of intracellular cAMP formation^[2]. Forskolin induces differentiation of various cell types and activates pregnane X receptor (PXR) and FXR^[3]. Forskolin exerts a inotropic effect on the heart, and has platelet antiaggregatory and antihypertensive actions. Forskolin also induces autophagy^[4]^[5].

IC₅₀ & Target

IC50: 41 nM (Adenylyl cyclase)^[1]
EC50: 0.5 μM (Adenylyl cyclase)^[1]

体外実験

Forskolin (Coleonol) is also a potent exosome biogenesis and/or secretion activator in prostate cancer (PC) cells^[8].
Forskolin (Fsk) is a naturally occurring diterpene isolated from Coleus forskholii, directly activates adenylyl cyclase (AC) through its catalytic subunit to increase intracellular levels of cyclic adenosine monophosphate (cAMP)^[1].
Forskolin (Fsk) affects the proliferation of the human T-cell lines such as Kit 225 and MT-2. Forskolin treatment inhibits the proliferation of both Kit 225 and MT-2 cells in a dose-dependent manner with an IC₅₀ equal to ~5 μM Fsk. Forskolin treatment (10-100 μM) increases cAMPi levels ~5- to 20-fold above basal levels, which reache maximum levels between 50-100 μM Forskolin^[6].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

The Forskolin (Coleonol)-treated Mrp4^-/- mice shows an increased number of Ki67-positive and cleaved caspase 3-positive ECs, a significant decrease in the amount of pericyte coverage, and a reduced number of empty sleeves. In pups exposed to hyperoxia (75% oxygen) from P7 to P12, the Mrp4^-/- mice shows a significant increase in the unvascularized retinal area^[2].
The average blood glucose in the healthy rat group is 102.12±1.94 mg/dL, 101.25±3.56 for control group and 103±2.08 in forskolin group. The data shows that glucose levels at the end of the study are lower in forskolin group, with a significant difference according to the statistical tests applied (p=0.03)^[7].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

410.50

Appearance

Solid

分子式

C₂₂H₃₄O₇

CAS 番号

66575-29-9

SMILES

O=C1[[email protected]@]2(O)[[email protected]]([[email protected]@H](OC(C)=O)[[email protected]@H](O)[[email protected]@]3([H])C(C)(C)CC[[email protected]](O)[[email protected]@]32C)(C)O[[email protected]@](C)(C=C)C1

Structure Classification

Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (243.61 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4361 mL	12.1803 mL	24.3605 mL
5 mM	0.4872 mL	2.4361 mL	4.8721 mL
10 mM	0.2436 mL	1.2180 mL	2.4361 mL

*Please refer to the solubility information to select the appropriate solvent.

体内:

1.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

*All of the co-solvents are available by MCE.

純度とドキュメンテーション

純度: 99.78%

Select Batch:

データシート (293 KB) SDS (393 KB)

COA (265 KB) HNMR (273 KB) LCMS (96 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Robbins JD, et al. Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J Med Chem. 1996 Jul 5;39(14):2745-52. [Content Brief]

[2]. Matsumiya W, et al. Forskolin modifies retinal vascular development in Mrp4-knockout mice. Invest Ophthalmol Vis Sci. 2012 Dec 7;53(13):8029-35. [Content Brief]

[3]. Mayati A, et al. Functional polarization of human hepatoma HepaRG cells in response to forskolin. Sci Rep. 2018 Oct 31;8(1):16115. [Content Brief]

[4]. Awad JA, et al. Interactions of forskolin and adenylate cyclase. Effects on substrate kinetics and protection against inactivation by heat and N-ethylmaleimide. J Biol Chem. 1983 Mar 10;258(5):2960-5. [Content Brief]

[5]. Seamon KB, et al. Structure-activity relationships for activation of adenylate cyclase by the diterpene forskolin and its derivatives. J Med Chem. 1983 Mar;26(3):436-9. [Content Brief]

[6]. Ríos-Silva M, et al. Effect of chronic administration of forskolin on glycemia and oxidative stress in rats with and without experimental diabetes. Int J Med Sci. 2014 Mar 11;11(5):448-52. [Content Brief]

[7]. Rodriguez G, et al. Forskolin-inducible cAMP pathway negatively regulates T-cell proliferation by uncoupling the interleukin-2 receptor complex. J Biol Chem. 2013 Mar 8;288(10):7137-46. [Content Brief]

[8]. Amrita Datta, et al. High-throughput screening identified selective inhibitors of exosome biogenesis and secretion: A drug repurposing strategy for advanced cancer. Sci Rep. 2018 May 25;8(1):8161. [Content Brief]

細胞実験 ^[2]	Quiescent Kit 225 or MT-2 cells are seeded into 96-well plates at 5×10⁴ cells per well. Cells are then pretreated for 1 h with 1% DMSO (vehicle) or Forskolin at 1, 5, 10, 25, 50, and 100 μMconcentrations. The cells are stimulated with IL-2 and cultured for an additional 20 h at 37°C. Control cells are treated with 1% DMSO for 20 h. During the final 4 h of incubation, the cells are pulsed with [³H]thymidine at a concentration of 0.5 μCi/200 μL. Cells are harvested onto fiberglass filters and analyzed using liquid scintillation counting^[2]. MCE はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[3]^[4]	Mice^[3] C57BL/6J mice are used. Mrp4-knockout mice, which are established and repeatedly backcrossed to the C57BL/6J mice. Forskolin is injected intraperitoneally into neonatal mice at postnatal days 4 (P4) and 5 (P5). Mice injected with DMSO serve as the controls. The treated mice are euthanized at P6, and their retinas are isolated for whole-mount immunohistochemistry (IHC). The effect of different concentrations of Forskolin on the survival rate and retinal vasculature is first tested, and the optimal concentration is determined, 1.0 μg/50 μL (0.3 mg/kg) at P4 and 1.5 μg/50 μL (0.5 mg/kg) at P5, used to compare the retinal vascular phenotypes between WT mice and Mrp4-deficient mice. Rats^[4] Male Wistar rats, aged 10-14 weeks old, with a mean weight of 300 g±50 g, are divided into four groups; 19 are experimentally induced to develop diabetes, and 8 are maintained in a healthy condition. Both diabetic and healthy rats receive no Forskolin (control), or 6 mg/kg per day of Forskolin, administered orally for 8 weeks. Blood glucose levels are determined in each group before and after Forskolin treatment. The diabetic rats are tested two weeks after confirming the presence of diabetes (three weeks after the induction) and after eight weeks of the designated treatment. MCE はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Robbins JD, et al. Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J Med Chem. 1996 Jul 5;39(14):2745-52. [Content Brief] [2]. Matsumiya W, et al. Forskolin modifies retinal vascular development in Mrp4-knockout mice. Invest Ophthalmol Vis Sci. 2012 Dec 7;53(13):8029-35. [Content Brief] [3]. Mayati A, et al. Functional polarization of human hepatoma HepaRG cells in response to forskolin. Sci Rep. 2018 Oct 31;8(1):16115. [Content Brief] [4]. Awad JA, et al. Interactions of forskolin and adenylate cyclase. Effects on substrate kinetics and protection against inactivation by heat and N-ethylmaleimide. J Biol Chem. 1983 Mar 10;258(5):2960-5. [Content Brief] [5]. Seamon KB, et al. Structure-activity relationships for activation of adenylate cyclase by the diterpene forskolin and its derivatives. J Med Chem. 1983 Mar;26(3):436-9. [Content Brief] [6]. Ríos-Silva M, et al. Effect of chronic administration of forskolin on glycemia and oxidative stress in rats with and without experimental diabetes. Int J Med Sci. 2014 Mar 11;11(5):448-52. [Content Brief] [7]. Rodriguez G, et al. Forskolin-inducible cAMP pathway negatively regulates T-cell proliferation by uncoupling the interleukin-2 receptor complex. J Biol Chem. 2013 Mar 8;288(10):7137-46. [Content Brief] [8]. Amrita Datta, et al. High-throughput screening identified selective inhibitors of exosome biogenesis and secretion: A drug repurposing strategy for advanced cancer. Sci Rep. 2018 May 25;8(1):8161. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Forskolin (Synonyms: Coleonol; Colforsin; HL 362)

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Forskolin (Synonyms: Coleonol; Colforsin; HL 362)

カスタマーレビュー

顧客検証

おすすめ

•関連スクリーニングライブラリ:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

モル濃度カルキュレーター

希釈カルキュレーター

The molarity calculator equation

The dilution calculator equation

Keywords:

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