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Furimazine (Synonyms: PBI 3939)

Cat. No.: HY-111497 Purity: 98.35%
Handling Instructions

Furimazine is an imidazopyrazinone substrate. NanoLuc (Nluc) paired with Furimazine produced 2.5 million-fold brighter luminescence in mammalian cells relative to Oluc-19 with Coelenterazine.

For research use only. We do not sell to patients.

Furimazine Chemical Structure

Furimazine Chemical Structure

CAS No. : 1374040-24-0

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5 mg USD 550 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Furimazine is an imidazopyrazinone substrate. NanoLuc (Nluc) paired with Furimazine produced 2.5 million-fold brighter luminescence in mammalian cells relative to Oluc-19 with Coelenterazine.

In Vitro

Furimazine is a Coelenterazine analogue.The apparent Km for purified NanoLuc (Nluc) using either Furimazine or Coelenterazine is ~10 μM, while the maximum luminescence (i.e., apparent Vmax) is ~30-fold higher for Furimazine than for native Coelenterazine. Measurement of luminescence intensity from cells expressing Nluc reveals that maximal signal is attained at about 10-20 μM Furimazine[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

381.43

Formula

C₂₄H₁₉N₃O₂

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (262.17 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6217 mL 13.1086 mL 26.2171 mL
5 mM 0.5243 mL 2.6217 mL 5.2434 mL
10 mM 0.2622 mL 1.3109 mL 2.6217 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.45 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[1]

Random libraries are generated by error-prone PCR (average of 2-3 mutations per clone). Library 1 (phase 1; template=Oluc-N166R) is screened (4,400 variants) with Coelenterazine. Library 2 (phase 2; template=C1A4E) is screened (4,400 variants) with 11 novel Coelenterazine analogues: 3840, 3841, 3842, 3857, 3880, 3881, 3886, 3887, 3889, 3897, and 3900. The 11 analogues represent substitutions at positions 2, 6, and 8 and are considered to be representative of the entire set of 24 compounds; 2,200 variants are screened with compounds 3896 and 3894. All hits (improved luminescence) are screened again with the remaining Coelenterazine analogues. Library 3 (phase 3; template=C1A4E+Q18L/K33N/F54I/F68Y/L72Q/M75K/I90V) was screened in the context of a mouse Id-X-HaloTag (where X=library) using Coelenterazine and Furimazine. Library screens are performed on a Freedom robotic workstation as follows: induced bacterial cultures (in 96-well microtiter plates) are lysed with a buffer containing 300 mM HEPES pH 8, 200 mM thiourea, 0.3X Passive Lysis Buffer, 0.3 mg/mL lysozyme, and 0.002 units of RQ1 DNase. Assay reagent containing 1 mM CDTA, 150 mM KCl, 10 mM DTT, 0.5% (v/v) Tergitol, and 20 μM substrate is then added to equal volumes of lysate. Samples are measured on a GENios Pro luminometer[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 98.35%

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Product Name:
Furimazine
Cat. No.:
HY-111497
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