2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA Stain

DNA Stain

DNA Stain

Related Products

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There are several different stains that can be used to visualize and photograph DNA after separation by gel electrophoresis. Molecules of the dye adhere to DNA strands and fluoresce under UV light, showing where the bands are within the gel.

Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra.

DNA Stain Related Products (69):

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D0814
    DAPI dihydrochloride
    		99.27%
    DAPI dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells.
    DAPI dihydrochloride
  • HY-101879
    Acridine Orange hydrochloride
    		99.86%
    Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).
    Acridine Orange hydrochloride
  • HY-D0021
    Ethidium bromide
    		99.85%
    Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
    Ethidium bromide
  • HY-110251
    DFHBI-1T
    		98.12%
    DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells.
    DFHBI-1T
  • HY-D0971
    Pyronin Y
    Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells.
    Pyronin Y
  • HY-D2220
    SiR-Hoechst
    SiR-Hoechst is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells. SiR-Hoechst is minimally toxic at concentrations as high as 10-25 µM. SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 µM.
    SiR-Hoechst
  • HY-D1686B
    Biotin-16-UTP tetrasodium
    Biotin-16-UTP tetrasodium is an active substrate for RNA polymerase. Biotin-16-UTP tetrasodium can replace UTP in the in vitro transcription reaction for RNA labeling.
    Biotin-16-UTP tetrasodium
  • HY-D1373
    HBC
    		99.69%
    HBC is a green fluorescent protein (GFP) fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC is used to detect RNA localization.
    HBC
  • HY-139912
    Biotin-aniline
    		98.41%
    Biotin-aniline is a probe with substantially high reactivity towards RNA and DNA. Biotin-aniline emerges as more efficient probe for capturing subcellular transcriptome in living cells with high spatial specificity.
    Biotin-aniline
  • HY-D1020
    7-Aminoactinomycin D
    		≥99.0%
    7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects.
    7-Aminoactinomycin D
  • HY-138200
    Cy5 maleimide
    		99.43%
    Cy5 maleimide is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cy5 maleimide
  • HY-W012642
    2-Aminopurine
    		99.53%
    2-Aminopurine, a fluorescent analog of guanosine and adenosine, is a widely used fluorescence-decay-based probe of DNA structure. When 2-Aminopurine is inserted in anoligonucleotide, its fluorescence is highly quenched by stacking with the natural bases. 2-Aminopurine has been used to probe nucleic acid structure and dynamics.
    2-Aminopurine
  • HY-D0723
    5(6)-TAMRA SE
    5(6)-TAMRA SE is the amine-reactive, mixed isomer form of TAMRA, which is a dye for oligonucleotide labeling and automated DNA sequencing applications.
    5(6)-TAMRA SE
  • HY-D0996
    LDS-751
    		99.30%
    Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751.
    LDS-751
  • HY-D1571
    CellTracker Blue CMF2HC Dye
    		99.78%
    CellTracker Blue CMF2HC Dye is a blue dye, can be used in two-channel nuclei acid sequencing, with blue and purple excitation light (450-460 nm/400-405nm or 415-450 nm/480-525nm). CellTracker Blue CMF2HC Dye can be used to rapid determination of antibiotic sensitivity of microorganisms.
    CellTracker Blue CMF2HC Dye
  • HY-D0925A
    Cy5.5-SE (DIPEA)
    Cy5.5-SE (Cyanine5.5 NHS ester) DIPEA is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance. Storage: protect from light.
    Cy5.5-SE (DIPEA)
  • HY-D0093
    Ethidium homodimer
    		≥99.0%
    Ethidium homodimer (EthD-1) is a high-affinity fluorescent nucleic acid dye commonly used to stain mammals, bacteria, yeast, and fungi. Ethidium homodimer binds to DNA or RNA, enhancing fluorescence more than 30 times. The Ethidium homodimer has a strong positive charge, so it cannot cross cell membranes and stain living cells; But the Ethidium homodimer can cross the disordered region of the dead cell membrane to reach the nucleus and embed the DNA double strand to produce red fluorescence. Therefore, Ethidium homodimer is a relatively sensitive nucleic acid stain that can accurately detect nucleic acids in solution or in decomposing cells. Ethidium homodimer binds DNA, Ex/Em=528/617 nm.
    Ethidium homodimer
  • HY-D0049
    6-TAMRA-SE
    6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing.
    6-TAMRA-SE
  • HY-136247
    Cyanine 5 Tyramide
    Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.
    Cyanine 5 Tyramide
  • HY-15623
    Hoechst 33258 analog
    		99.96%
    Hoechst 33258 analog is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    Hoechst 33258 analog