1. Cytoskeleton
  2. Myosin
  3. (-)-Blebbistatin

(-)-Blebbistatin (Synonyms: (S)-(-)-Blebbistatin)

製品番号: HY-13441 純度: 99.42%
取扱説明書

(-)-Blebbistatin is a selective inhibitor of the ATPase activity of non-muscle myosin II.

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(-)-Blebbistatin 構造式

(-)-Blebbistatin 構造式

CAS 番号 : 856925-71-8

容量 価格(税別) 在庫状況 数量
5 mg USD 72 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 120 在庫あり
Estimated Time of Arrival: December 31
50 mg USD 420 在庫あり
Estimated Time of Arrival: December 31
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200 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

カスタマーレビュー

Based on 4 publication(s) in Google Scholar

Other Forms of (-)-Blebbistatin:

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製品説明

(-)-Blebbistatin is a selective inhibitor of the ATPase activity of non-muscle myosin II[1][2].

IC50 & Target

IC50: 0.5 to 5 μM (myosin II)[1]

体外実験

Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. Smooth muscle myosin is only poorly inhibited (IC50=80 μM)[1]. Blebbistatin does not compete with nucleotide binding to the skeletal muscle myosin subfragment-1. The inhibitor preferentially binds to the ATPase intermediate with ADP and phosphate bound at the active site, and it slows down phosphate release. It blocks the myosin heads in a products complex with low actin affinity[2]. In culture-activated hepatic stellate cells, blebbistatin is found to change both cell morphology and function. Stellate cells become smaller, acquire a dendritic morphology and have less myosin IIA-containing stress fibres and vinculin-containing focal adhesions. Blebbistatin impairs silicone wrinkle formation, reduces collagen gel contraction and blocks endothelin-1-induced intracellular Ca2+ release. It promotes wound-induced cell migration[3].

体内実験

Blebbistatin dose-dependently and completely relax both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4].

分子量

292.33

分子式

C₁₈H₁₆N₂O₂

CAS 番号

856925-71-8

SMILES

CC1=CC=C(N=C(N(C2=CC=CC=C2)CC3)[[email protected]]3(O)C4=O)C4=C1

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

溶剤 & 溶解度
体外: 

DMSO : 10 mg/mL (34.21 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4208 mL 17.1040 mL 34.2079 mL
5 mM 0.6842 mL 3.4208 mL 6.8416 mL
10 mM 0.3421 mL 1.7104 mL 3.4208 mL
*Please refer to the solubility information to select the appropriate solvent.
体内:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1 mg/mL (3.42 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 1 mg/mL (3.42 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1 mg/mL (3.42 mM); Clear solution

*All of the co-solvents are provided by MCE.
参考文献
細胞実験
[3]

Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3].

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

参考文献

純度: 99.42%

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Keywords:

(-)-Blebbistatin(S)-(-)-BlebbistatinMyosinInhibitorinhibitorinhibit

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製品名:
(-)-Blebbistatin
製品番号:
HY-13441
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