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  3. Capsaicinoid

Capsaicinoid is a mixture of Capsaicin and Dihydrocapsaicin. Capsaicinoid is an capsaicin receptor (TRPV1) agonist.

For research use only. We do not sell to patients.

Capsaicinoid Chemical Structure

Capsaicinoid Chemical Structure

CAS No. : 404-86-4

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Solid + Solvent
10 mM * 1 mL in DMSO
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Customer Review

Based on 46 publication(s) in Google Scholar

Other Forms of Capsaicinoid:

Top Publications Citing Use of Products

43 Publications Citing Use of MCE Capsaicinoid

WB
Cell Viability Assay

    Capsaicinoid purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Jun 24;10(17):7906-7920.  [Abstract]

    The expression of cell cycle-associated molecules is altered by Capsaicin treatment in both cell lines.The cell-cycle inhibitor p27Kip1 is upregulated after Capsaicin treatment while the G1-S phase transition marker cyclin D1 is downregulated. p38 phosphorylation levels are also decreased following capsaicin treatment.

    Capsaicinoid purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Jun 24;10(17):7906-7920.  [Abstract]

    CNE2 and SUNE1 cell growth is inhibited by Capsaicin in a dose-dependent manner.

    Capsaicinoid purchased from MedChemExpress. Usage Cited in: Clin Exp Pharmacol Physiol. 2017 Jul;44(7):803-814.  [Abstract]

    Western blot analysis showing that the CAP (0.5 mg/kg) injection inhibits the increase of p65 expression and phosphorylation at 24 h after I/R injury.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Capsaicinoid is a mixture of Capsaicin and Dihydrocapsaicin. Capsaicinoid is an capsaicin receptor (TRPV1) agonist[1][2].

    In Vitro

    Capsaicin is the main Capsaicinoid in chili peppers, followed by Dihydrocapsaicin. These two compounds provide about twice hotness to the taste and nerves as the minor capsaicinoids[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: Human pharyngeal squamous carcinoma cells (FaDu) cells
    Concentration: 50 µM, 100 µM, 200 µM, and 300 µM
    Incubation Time: 24 hours, 48 hours and 72 hours
    Result: Showed an augmented decrease in cell growth.

    Apoptosis Analysis[2]

    Cell Line: FaDu cells
    Concentration: 50 µM, 100 µM and 200 µM
    Incubation Time: 12 hours
    Result: Increased the activity of caspase 3 in a time-dependent manner.

    Western Blot Analysis[2]

    Cell Line: FaDu cells
    Concentration: 200 µM
    Incubation Time: 24 hours
    Result: The observed activation of caspase 3 and PARP (p85) levels.
    In Vivo
    Animal Model: Swiss albino mice (20-25 g; 8-10 weeks old) induced by Benzo(a)pyrene[3]
    Dosage: 10 mg/kg
    Administration: Intraperitoneal administration; once in a week; for 14 weeks
    Result: Inhibited the development of mice lung carcinogenesis.
    Clinical Trial
    Molecular Weight

    305.41

    Formula

    C18H27NO3

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC(C)/C=C/CCCCC(NCC1=CC=C(O)C(OC)=C1)=O

    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (327.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2743 mL 16.3714 mL 32.7429 mL
    5 mM 0.6549 mL 3.2743 mL 6.5486 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.46%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.2743 mL 16.3714 mL 32.7429 mL 81.8572 mL
    5 mM 0.6549 mL 3.2743 mL 6.5486 mL 16.3714 mL
    10 mM 0.3274 mL 1.6371 mL 3.2743 mL 8.1857 mL
    15 mM 0.2183 mL 1.0914 mL 2.1829 mL 5.4571 mL
    20 mM 0.1637 mL 0.8186 mL 1.6371 mL 4.0929 mL
    25 mM 0.1310 mL 0.6549 mL 1.3097 mL 3.2743 mL
    30 mM 0.1091 mL 0.5457 mL 1.0914 mL 2.7286 mL
    40 mM 0.0819 mL 0.4093 mL 0.8186 mL 2.0464 mL
    50 mM 0.0655 mL 0.3274 mL 0.6549 mL 1.6371 mL
    60 mM 0.0546 mL 0.2729 mL 0.5457 mL 1.3643 mL
    80 mM 0.0409 mL 0.2046 mL 0.4093 mL 1.0232 mL
    100 mM 0.0327 mL 0.1637 mL 0.3274 mL 0.8186 mL
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Capsaicinoid
    Cat. No.:
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