1. Apoptosis
    Metabolic Enzyme/Protease
    Cell Cycle/DNA Damage
  2. MDM-2/p53
    HSP
  3. Colletofragarone A2

Colletofragarone A2 

Cat. No.: HY-N10502
Handling Instructions

Colletofragarone A2 can be be isolated from the fungus Colletotrichum sp. (13S020). Colletofragarone A2 inhibits mutant p53 and HSP90 with anti-cancer activity. Colletofragarone A2 promotes degradation and aggregation of mutant p53 and suppressing tumor growth in vivo.

For research use only. We do not sell to patients.

Colletofragarone A2 Chemical Structure

Colletofragarone A2 Chemical Structure

Size Stock
100 mg   Get quote  
250 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

View All HSP Isoform Specific Products:

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Colletofragarone A2 can be be isolated from the fungus Colletotrichum sp. (13S020). Colletofragarone A2 inhibits mutant p53 and HSP90 with anti-cancer activity. Colletofragarone A2 promotes degradation and aggregation of mutant p53 and suppressing tumor growth in vivo[1].

IC50 & Target

HSP90

 

In Vitro

Mutant p53 loses original tumor suppressor function but acquires new abilities regarding oncogenic progression[1].
Colletofragarone A2 (0.05-5 μM; 72 h) shows high selectivity and more cytotoxic activity on cells with p53R175H structural mutants than with other p53 statuses such as a DNA-contact mutant, wild-type, and null cells[1].
Colletofragarone A2 (2 μM; 8 h) decreases mutant p53 levels in SK-BR-3 (p53R175H) cells by promoting p53 degradation[1].
Colletofragarone A2, combinded with 10 μM MG-132 (HY-13259), (2 or 4 μM; 4 h) induces the accumulation of the aggregated mutant p53[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: SK-BR-3 (p53R175H), HuCCT1 (p53R175H), Saos-2 (p53R175H), OVCAR-3 (p53R248Q), and A549 (wild-type p53)
Concentration: 0, 0.5, 1, 2, 4 μM
Incubation Time: 4 hours
Result: Decreased the mutant p53R175H levels in SK-BR-3 and HuCCT1 cells in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: SK-BR-3 (p53R175H) cells
Concentration: 2 μM, 4 μM (with 10 μM MG-132 (HY-13259), respectively)
Incubation Time: 4 hours
Result: Promoted the proteasome-mediated degradation of mutant p53 and the accumulation of precipitated mutant p53.

Cell Viability Assay[1]

Cell Line: SK-BR-3 (p53R175H), HuCCT1 (p53R175H), OVCAR-3 (p53R248Q), and A549 (wild-type p53) cells
Concentration: 0.05-5 μM
Incubation Time: 72 hours
Result: Decreased the level of p53 and showed high selectivity and more cytotoxic activity on cells with p53R175H structural mutants, IC50s of 0.18 μM (SK-BR-3), 0.35 μM (HuCCT1), respectively.
In Vivo

Colletofragarone A2 (0.35 mM, 100 μL; injected intratumorally and daily; 13 d) markedly decreases tumor cell growth in mouse infected with HuCCT1 (p53R175H) cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Xenograft model with HuCCT1 (p53R175H) cells in mouse[1]
Dosage: 0.35 mM with 100 μL (DMSO solution)
Administration: Injected intratumorally; daily; 13 days; tested tumor growth at 1, 3, 5, 7, 9, 13 days
Result: Inhibited tumor growth without lowing body weight of mouse.
Molecular Weight

386.44

Formula

C22H26O6

SMILES

C/C=C/C=C/C=C/C1=C2[[email protected]@]3([H])[[email protected]@](C=CC([[email protected]@]3([H])[[email protected]](O)C[[email protected]](O)C[[email protected]](C)OC2=O)=O)([H])O1

Structure Classification
Source

Colletotrichum sp.

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Colletofragarone A2
Cat. No.:
HY-N10502
Quantity:
MCE Japan Authorized Agent: