Curzerene

Cat. No.: HY-N1963 Purity: ≥97.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Curzerene is a sesquiterpene is isolated from the rhizome of Curculigo orchioides Gaertn with anti-cancer activity. Curzerene inhibits glutathione S-transferase A1 (GSTA1) mRNA and protein expression. Curzerene induces cell apoptosis.

For research use only. We do not sell to patients.

Curzerene Chemical Structure

CAS No. : 17910-09-7

Size	Price	Stock	Quantity
1 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
1 mg * 5	USD 176	In-stock	Estimated Time of Arrival: December 31
1 mg * 10	USD 319	In-stock	Estimated Time of Arrival: December 31
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10 mg		Get quote

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Biological Activity
Purity & Documentation
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Description

IC₅₀ & Target

GSTA1^[1]

In Vitro

Curzerene (0-100 µM; 24-72 hours) indicates that cell inhibition increases in a dose- and time-dependent manner, IC₅₀ to SPC A1 cells at 24, 48, and 72 h was 403.8 μM, 154.8 µM, and 47.01 µM, respectively^[1].
Curzerene (0-100 µM; 48 hours) exhibits a higher percentage of apoptotic and necrotic cells than that of the control group in SPC-A1cells^[1].
Curzerene(0-100 µM; 48 hours) indicates that the percentage of cells arrested in the G2/M phase increased from 9.26% in the control group cells to 17.57% in the cells treated with the highest dose^[1].
Curzerene (6.25-100 µM; 48 hours) decreases the mRNA expression of GSTA1 in SPC A1 cells^[1].
Curzerene (6.25-100 µM; 48 hours) decreases the protein expression of GSTA1 in SPC A1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	SPC-A1 cells
Concentration:	0 µM, 6.25 µM, 12.5 µM, 25 µM, 50 µM, 100 µM
Incubation Time:	24 hours, 48 hours, 72 hours
Result:	Inhibited growth of non-small cell lung cancer SPC A1 cells in vitro.

Apoptosis Analysis^[1]

Cell Line:	SPC-A1 cells
Concentration:	0 µM, 6.25 µM, 12.5 µM, 25 µM, 50 µM, 100 µM
Incubation Time:	48 hours
Result:	Induced apoptosis of the cells in a dose-dependent manner.

Apoptosis Analysis^[1]

Cell Line:	SPC-A1 cells
Concentration:	0 µM, 6.25 µM, 12.5 µM, 25 µM, 50 µM, 100 µM
Incubation Time:	48 hours
Result:	Induced G2/M cell cycle arrest of SPC A1 cells.

RT-PCR^[1]

Cell Line:	SPC-A1 cells
Concentration:	6.25 µM, 25 µM, 100 µM
Incubation Time:	48 hours
Result:	Decreased GSTA1 mRNA expression.

Molecular Weight

216.32

Formula

C₁₅H₂₀O

CAS No.

17910-09-7

Appearance

Liquid

Color

Colorless to light yellow

SMILES

C=C([C@H]([C@@](C)(C=C)C1)CC2=C1OC=C2C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (1155.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.6228 mL	23.1139 mL	46.2278 mL
5 mM	0.9246 mL	4.6228 mL	9.2456 mL
10 mM	0.4623 mL	2.3114 mL	4.6228 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (28.89 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 6.25 mg/mL (28.89 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 6.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 6.25 mg/mL (28.89 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥97.0%

Select Batch:

Data Sheet (252 KB) SDS (252 KB)

COA (254 KB) HNMR (187 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.6228 mL	23.1139 mL	46.2278 mL	115.5695 mL
	5 mM	0.9246 mL	4.6228 mL	9.2456 mL	23.1139 mL
	10 mM	0.4623 mL	2.3114 mL	4.6228 mL	11.5570 mL
	15 mM	0.3082 mL	1.5409 mL	3.0819 mL	7.7046 mL
	20 mM	0.2311 mL	1.1557 mL	2.3114 mL	5.7785 mL
	25 mM	0.1849 mL	0.9246 mL	1.8491 mL	4.6228 mL
	30 mM	0.1541 mL	0.7705 mL	1.5409 mL	3.8523 mL
	40 mM	0.1156 mL	0.5778 mL	1.1557 mL	2.8892 mL
	50 mM	0.0925 mL	0.4623 mL	0.9246 mL	2.3114 mL
	60 mM	0.0770 mL	0.3852 mL	0.7705 mL	1.9262 mL
	80 mM	0.0578 mL	0.2889 mL	0.5778 mL	1.4446 mL
	100 mM	0.0462 mL	0.2311 mL	0.4623 mL	1.1557 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Curzerene17910-09-7Inhibitorinhibitorinhibit

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