2. Cell Cycle/DNA Damage Autophagy Anti-infection Apoptosis
  3. CDK Autophagy HIV Apoptosis
  4. Flavopiridol

Flavopiridol  (Synonyms: HMR-1275; Alvocidib; L86-8275)

Cat. No.: HY-10005 Purity: 99.72%
COA Handling Instructions

Flavopiridol (Alvocidib) is a broad spectrum and competitive inhibitor of CDKs, inhibiting CDK1, CDK2, CDK4 with IC50s of 30, 170, 100 nM, respectively.

For research use only. We do not sell to patients.

Flavopiridol Chemical Structure

Flavopiridol Chemical Structure

CAS No. : 146426-40-6

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 73 In-stock
Solid
5 mg USD 66 In-stock
10 mg USD 92 In-stock
50 mg USD 257 In-stock
100 mg USD 475 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 32 publication(s) in Google Scholar

Other Forms of Flavopiridol:

Flavopiridol Related Antibodies

Top Publications Citing Use of Products

30 Publications Citing Use of MCE Flavopiridol

RT-PCR
Proliferation Assay

    Flavopiridol purchased from MCE. Usage Cited in: Sci Rep. 2015 Dec 1;5:17675.  [Abstract]

    Flavopiridol (FP) significantly decreases renilla luciferase mRNA levels time-dependently. The bar graphs (upper panel) represent renilla luciferase mRNA levels in pRL-SV40-transfected A549 cells treated with FP normalized to that in FP free cells. The bottom panel indicates renilla luciferase mRNA levels determined by RT-PCR using 1 μg of total RNA as template.

    Flavopiridol purchased from MCE. Usage Cited in: Biomaterials. 2014 Aug;35(24):6585-94.  [Abstract]

    Flavopiridol at concentrations >0.1 μM significantly inhibits the proliferation of astrocytes, and at ≥1 μM even inhibits their survival.

    View All CDK Isoform Specific Products:

    View All Isoforms
    CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Flavopiridol (Alvocidib) is a broad spectrum and competitive inhibitor of CDKs, inhibiting CDK1, CDK2, CDK4 with IC50s of 30, 170, 100 nM, respectively.

    IC50 & Target[3]

    CDK1/Cyc B1

    30 nM (IC50)

    CDK2/Cyc E

    170 nM (IC50)

    CDK4/Cyc D1

    100 nM (IC50)

    MAP

    19000 nM (IC50)

    PKC

    14000 nM (IC50)

    EGFR

    22000 nM (IC50)

    In Vitro

    Flavopiridol (2 μM) robustly induces a distinct pattern of ER stress in CLL cells that contributes to cell death through IRE1-mediated activation of ASK1 and possibly downstream caspases[1]. Flavopiridol results in potent upregulation of a number of PRGs in treatments lasting 4-24 h. Flavopiridol has and immediate and long-term effect on the expression of several PRGs. In serum starved cells re-stimulated with serum, flavopiridol also inhibits the expression of these genes, but subsequently, JUNB, GADD45B and EGR1 are upregulated in the presence of flavopiridol[2].
    Flavopiridol (Alvocidib) also inhibits cyclin E1 and induces apoptosis[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Flavopiridol Related Antibodies
    Clinical Trial
    Molecular Weight

    401.84

    Appearance

    Solid

    Formula

    C21H20ClNO5
    CAS No.
    SMILES

    O=C1C2=C(C=C(C([C@]3([H])[C@H](O)CN(C)CC3)=C2OC(C4=CC=CC=C4Cl)=C1)O)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, stored under nitrogen

    *In solvent : -80°C, 2 years; -20°C, 1 year (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (82.94 mM; Need ultrasonic)

    DMSO : 1 mg/mL (2.49 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4886 mL 12.4428 mL 24.8855 mL
    5 mM 0.4977 mL 2.4886 mL 4.9771 mL
    10 mM 0.2489 mL 1.2443 mL 2.4886 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.22 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.22 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.72%

    COA (255 KB) HNMR (194 KB) LCMS (121 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    The cells treated with flavopiridol are washed after 4 hours with PBS and resuspended in regular growth medium (RPMI 1640) supplemented with 10% human serum and antibiotics for the remainder of the incubation time. In the case of flavopiridol/NSC-187208 samples, NSC-187208 is re-added in the fresh media after flavopiridol is washed at 4 hours. For all the other conditions, cells are incubated with the respective drugs for 24 hours continuously.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Flavopiridol Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Flavopiridol146426-40-6HMR-1275 Alvocidib L86-8275HMR1275HMR 1275CDKAutophagyHIVApoptosisCyclin dependent kinaseHuman immunodeficiency virusInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Salutation

    Applicant Name *

    		 

    Email Address *

    Phone Number *

    		 

    Organization Name *

    Department *

    		 

    Requested quantity *

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Flavopiridol
    Cat. No.:
    HY-10005
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.