2. Epigenetics
    TGF-beta/Smad
  3. PKC
  4. Go 6983

Go 6983  (Synonyms: Gö 6983; Goe 6983)

Cat. No.: HY-13689 Purity: 98.42%
COA Handling Instructions

Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively.

For research use only. We do not sell to patients.

Go 6983 Chemical Structure

Go 6983 Chemical Structure

CAS No. : 133053-19-7

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Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 117 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 117 In-stock
Solid
5 mg USD 106 In-stock
10 mg USD 172 In-stock
50 mg USD 541 In-stock
100 mg USD 911 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 48 publication(s) in Google Scholar

Top Publications Citing Use of Products

42 Publications Citing Use of MCE Go 6983

WB
RT-PCR
IF

    Go 6983 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Feb 7;9(2):165.  [Abstract]

    Western blot analysis of MCF-7 cells treated with Tan IIA at the specified concentrations for the expression of cytosolic PKC isoforms. The compound Go 6983 (GO, 20 μM) is included as a positive control.

    Go 6983 purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 3;503(1):62-70.  [Abstract]

    BMMs are incubated in serum-free medium and pretreated with or without Go6983 (0, 2.5 μM) for 4 h. Next, the BMMs are stimulated by RANKL (50 ng/mL) for the indicated times (0, 15, 30, 60 min). Additionally, the BMM lysates are analyzed by western blotting.

    Go 6983 purchased from MCE. Usage Cited in: Vet Microbiol. 2016 Aug 30;192:26-33.  [Abstract]

    Effect of PKC inhibitor on NF-kB DNA binding activity and NFAT inhibitor on NFATc1 DNA binding activity. Lane N is negative control; lane 1 is control group; lane 2 is S. uberis group; lane 3 is pre-treated with 2 μM Go 6983; lane 4 is pre-treated with 5 μM Go 6983; lane 5 is pre-treated with 10 μM Go 6983.

    Go 6983 purchased from MCE. Usage Cited in: J Appl Toxicol. 2016 Nov;36(11):1409-17.   [Abstract]

    Protein levels of GLT-1 analyzed by Western blotting in C6 cells then treated with TCDD for 12 h. Cells are pre-treated with Ca2+-signaling blocker BAY-a-1040 (25 nM) for 30min before TCDD stimulation. The PKC inhibitor staurosporine (10 nM) and Go 6983 (1 μM) is applied 2 h before adding TCDD.

    Go 6983 purchased from MCE. Usage Cited in: J Appl Toxicol. 2016 Nov;36(11):1409-17.   [Abstract]

    Quantitative analysis of the intensity of GLT-1 expression relative to GAPDH in the indicated groups. Cells are pre-treated with Ca2+-signaling blocker BAY-a-1040 (25 nM) for 30min before TCDD stimulation. The PKC inhibitor staurosporine (10 nM) and Go 6983 (1 μM) is applied 2 h before adding TCDD.

    Go 6983 purchased from MCE. Usage Cited in: J Appl Toxicol. 2016 Nov;36(11):1409-17.   [Abstract]

    Immunofluorescence staining of PKC after pre-treatment with the Ca2+-signaling blocker and the PKC inhibitor. PKC is enriched in cytoplasmor perinucleus in untreated C6 cells (a-c), Go 6983-treated cells (g-i) or BAY-a-1040-treated cells (m-o). TCDD induces PKC translocation to the membrane in C6 glioma cells (d-f ).

    View All PKC Isoform Specific Products:

    View All Isoforms
    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKCι

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively.

    IC50 & Target[1]

    PKCγ

    6 nM (IC50)

    PKCα

    7 nM (IC50)

    PKCβ

    7 nM (IC50)

    PKCδ

    10 nM (IC50)

    PKCζ

    60 nM (IC50)

    PKCμ

    20000 nM (IC50)

    In Vitro

    Go 6983 inhibits PKCμ with IC50 of 20 μM, and the pther PKC isoenzymes can be suppressed by Go 6983 with IC50 values from 7 to 60 nM[1]. Go 6983 (100 nM) significantly reduces PMN adherence to the endothelium and infiltration into the myocardium compared with I/R + PMN hearts, and significantly inhibits superoxide release from PMNs by 90 +/- 2% in rat hearts[2]. Go 6983 (200 nM) has a reduced cardioprotective effect compared with the cardioprotective Go 6983 concentrations (50 and 100 nM) despite inhibiting PMN superoxide release by 99%[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    442.51

    Appearance

    Solid

    Formula

    C26H26N4O3
    CAS No.
    SMILES

    O=C(C(C1=CN(CCCN(C)C)C2=C1C=C(OC)C=C2)=C3C4=CNC5=C4C=CC=C5)NC3=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 34 mg/mL (76.83 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2598 mL 11.2992 mL 22.5984 mL
    5 mM 0.4520 mL 2.2598 mL 4.5197 mL
    10 mM 0.2260 mL 1.1299 mL 2.2598 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (5.65 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (5.65 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: 2.5 mg/mL (5.65 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 98.42%

    COA (256 KB) HNMR (437 KB) RP-HPLC (206 KB) LCMS (87 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    Phosphorylation reactions are carried out in a total volume of 100 μL, containing buffer C (50 mM Tris-HCl, pH 7.5, 10 mM β-mercaptoethanol), 4 mM MgCl2, 10 μg PS, 100 nM TPA, 5 μL of a Sf158 cell extract as a source of recombinant PKCμ or of Sf9 cell extracts as a source of other recombinant PKC isoenzymes, 10 μg of syntide 2 as substrate, and 35 μM ATP containing 1 μCi [γ-32P]ATP. In some experiments, PS and TPA are omitted or various inhibitors at concentrations indicated in the text are added. After incubation for 10 min at 30°C, the reaction is terminated by transferring 50 μL of the assay mixture onto a 20 mm square piece of phosphocellulose paper, which is washed 3 times in deionized water and twice in acetone. The radioactivity on each paper is determined by liquid scintillation counting.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    × = ×
    C1   V1   C2   V2

    Keywords:

    Go 6983133053-19-7Gö 6983 Goe 6983Go6983Go-6983Goe6983Goe 6983Goe-6983PKCProtein kinase CInhibitorinhibitorinhibit

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