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  4. HBC599

HBC599 is a HBC analog. HBC is nonfluorescent in solution, but emits strong fluorescence upon forming tight complex with Pepper RNA aptamer. HBC-Pepper complex can be used to visualize RNA dynamics in live cells.

For research use only. We do not sell to patients.

HBC599 Chemical Structure

HBC599 Chemical Structure

CAS No. : 2530162-00-4

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 220 In-stock
Solution
10 mM * 1 mL in DMSO USD 220 In-stock
Solid
5 mg USD 200 In-stock
10 mg USD 350 In-stock
25 mg USD 700 In-stock
50 mg USD 1100 In-stock
100 mg USD 1650 In-stock
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Based on 1 publication(s) in Google Scholar

HBC599 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

HBC599 is a HBC analog. HBC is nonfluorescent in solution, but emits strong fluorescence upon forming tight complex with Pepper RNA aptamer. HBC-Pepper complex can be used to visualize RNA dynamics in live cells[1].

In Vitro

Pepper can activate the fluorescence of HBC. Mammalian cells expressing Pepper exhibits robust greenyellowish fluorescence on incubation with 1 μM HBC[1].
Peppers allow simple and robust imaging of diverse RNA species in live cells with minimal perturbation of the target RNA’s transcription, localization and translation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HBC599 Related Antibodies
Molecular Weight

359.44

Formula

C21H17N3OS
CAS No.
Appearance

Solid

Color

Wine to reddish brown

SMILES

N#C/C(C1=CC=C(C#N)C=C1)=C\C2=CC3=CC=C(N(C)CCO)C=C3S2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 25 mg/mL (69.55 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7821 mL 13.9105 mL 27.8211 mL
5 mM 0.5564 mL 2.7821 mL 5.5642 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.75%

COA (245 KB) HNMR (144 KB) RP-HPLC (104 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7821 mL 13.9105 mL 27.8211 mL 69.5526 mL
5 mM 0.5564 mL 2.7821 mL 5.5642 mL 13.9105 mL
10 mM 0.2782 mL 1.3911 mL 2.7821 mL 6.9553 mL
15 mM 0.1855 mL 0.9274 mL 1.8547 mL 4.6368 mL
20 mM 0.1391 mL 0.6955 mL 1.3911 mL 3.4776 mL
25 mM 0.1113 mL 0.5564 mL 1.1128 mL 2.7821 mL
30 mM 0.0927 mL 0.4637 mL 0.9274 mL 2.3184 mL
40 mM 0.0696 mL 0.3478 mL 0.6955 mL 1.7388 mL
50 mM 0.0556 mL 0.2782 mL 0.5564 mL 1.3911 mL
60 mM 0.0464 mL 0.2318 mL 0.4637 mL 1.1592 mL
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HBC599 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HBC5992530162-00-4HBC 599HBC-599Fluorescent DyeHBC-likefluorophoreRNAtranscriptionlocalizationtranslationfluorescentPepperdynamicsInhibitorinhibitorinhibit

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