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HBC620 

Cat. No.: HY-133520 Purity: 99.32%
Handling Instructions

HBC620 is a HBC analog. HBC is nonfluorescent in solution, but emits strong fluorescence upon forming tight complex with Pepper RNA aptamer. HBC-Pepper complex can be used to visualize RNA dynamics in live cells.

For research use only. We do not sell to patients.

HBC620 Chemical Structure

HBC620 Chemical Structure

Size Price Stock Quantity
5 mg USD 300 In-stock
Estimated Time of Arrival: December 31
10 mg USD 480 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1400 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2200 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

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Description

HBC620 is a HBC analog. HBC is nonfluorescent in solution, but emits strong fluorescence upon forming tight complex with Pepper RNA aptamer. HBC-Pepper complex can be used to visualize RNA dynamics in live cells[1].

In Vitro

Pepper can activate the fluorescence of HBC. Mammalian cells expressing Pepper exhibits robust greenyellowish fluorescence on incubation with 1 μM HBC[1].
Peppers allow simple and robust imaging of diverse RNA species in live cells with minimal perturbation of the target RNA’s transcription, localization and translation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

365.47

Formula

C₁₉H₁₅N₃OS₂

SMILES

OCCN(C)C1=CC(S2)=C(S1)C=C2/C=C(C#N)/C3=CC=C(C#N)C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (45.61 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7362 mL 13.6810 mL 27.3620 mL
5 mM 0.5472 mL 2.7362 mL 5.4724 mL
10 mM 0.2736 mL 1.3681 mL 2.7362 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.08 mg/mL (5.69 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References
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Keywords:

HBC620HBC 620HBC-620OthersHBC-likefluorophoreRNAtranscriptionlocalizationtranslationfluorescentPepperdynamicsInhibitorinhibitorinhibit

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Product Name:
HBC620
Cat. No.:
HY-133520
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