Histamine-α,α,β,β-d₄ dihydrochloride (Synonyms: Ergamine-α,α,β,β-d₄ (dihydrochloride))

Name: Histamine-α,α,β,β-d 4 dihydrochloride
Brand: MedChemExpress
SKU: HY-B1204S
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1204S Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Histamine-α,α,β,β-d₄ (dihydrochloride) is the deuterium labeled Histamine. Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter.

For research use only. We do not sell to patients.

Histamine-α,α,β,β-d<sub>4</sub> dihydrochloride Chemical Structure

Histamine-α,α,β,β-d₄ dihydrochloride Chemical Structure

CAS No. : 344299-48-5

Size	Price	Stock	Quantity
1 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 810	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Histamine-α,α,β,β-d₄ dihydrochloride:

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Description

In Vitro

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

188.09

Formula

C₅H₇D₄Cl₂N₃

CAS No.

344299-48-5

Appearance

Solid

Color

Off-white to light yellow

SMILES

NC([2H])([2H])C([2H])([2H])C1=CN=CN1.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (132.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3166 mL	26.5830 mL	53.1660 mL
5 mM	1.0633 mL	5.3166 mL	10.6332 mL
10 mM	0.5317 mL	2.6583 mL	5.3166 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (257 KB)

Handling Instructions (2659 KB)

References

[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.3166 mL	26.5830 mL	53.1660 mL	132.9151 mL
	5 mM	1.0633 mL	5.3166 mL	10.6332 mL	26.5830 mL
	10 mM	0.5317 mL	2.6583 mL	5.3166 mL	13.2915 mL
	15 mM	0.3544 mL	1.7722 mL	3.5444 mL	8.8610 mL
	20 mM	0.2658 mL	1.3292 mL	2.6583 mL	6.6458 mL
	25 mM	0.2127 mL	1.0633 mL	2.1266 mL	5.3166 mL
	30 mM	0.1772 mL	0.8861 mL	1.7722 mL	4.4305 mL
	40 mM	0.1329 mL	0.6646 mL	1.3292 mL	3.3229 mL
	50 mM	0.1063 mL	0.5317 mL	1.0633 mL	2.6583 mL
	60 mM	0.0886 mL	0.4431 mL	0.8861 mL	2.2153 mL
	80 mM	0.0665 mL	0.3323 mL	0.6646 mL	1.6614 mL
	100 mM	0.0532 mL	0.2658 mL	0.5317 mL	1.3292 mL

Histamine-α,α,β,β-d₄ dihydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Histamine-α,α,β,β-d₄344299-48-5Ergamine-α,α,β,β-d₄Histamine ReceptorEndogenous MetaboliteInhibitorinhibitorinhibit

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Histamine-α,α,β,β-d4 dihydrochloride (Synonyms: Ergamine-α,α,β,β-d4 (dihydrochloride))

Customer Review

Other Forms of Histamine-α,α,β,β-d4 dihydrochloride:

Histamine-α,α,β,β-d4 dihydrochloride Related Antibodies

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•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Histamine-α,α,β,β-d4 dihydrochloride Related Antibodies

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Complete Stock Solution Preparation Table

Histamine-α,α,β,β-d4 dihydrochloride Related Classifications

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Histamine-α,α,β,β-d₄ dihydrochloride (Synonyms: Ergamine-α,α,β,β-d₄ (dihydrochloride))

Other Forms of Histamine-α,α,β,β-d₄ dihydrochloride:

Histamine-α,α,β,β-d₄ dihydrochloride Related Antibodies

Histamine-α,α,β,β-d₄ dihydrochloride Related Antibodies

Histamine-α,α,β,β-d₄ dihydrochloride Related Classifications