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  2. Rapid Running Buffer Powder (1 L of 1×)

Rapid Running Buffer Powder (1 L of 1×) 

Cat. No.: HY-K1017
Manual SDS

MCE Rapid Running Buffer Powder (1 L of 1×) is mainly used for agarose gel electrophoresis of nucleic acid.

Rapid Running Buffer Powder (1 L of 1×)
Size Price Stock
10 pouches USD 52 Ask For Quote & Lead Time
50 pouches USD 155 Ask For Quote & Lead Time

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Rapid Running Buffer (RRB) is mainly used for agarose gel electrophoresis of nucleic acid. DNA fragments (< 5 kb) can be separated effectively under RRB buffer. Combined with a certain concentration of agarose gel, RRB buffer can support high pressure fast electrophoresis at 300-350 V (15 V/cm), reducing time to about 10 minutes. RRB buffer is also an alternative to TBE buffer for agarose gel electrophoresis of small-sized DNA.

Each pouch of MCE Rapid Running Buffer Powder (1 L of 1×) can be diluted to 1 L 1× RRB buffer. This product provides a convenient way to make RRB buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1.   Prepare 1 L 1× RRB buffer

a.   Add 1 pouch of RRB Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

b.   Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× RRB buffer.

Note: The pH of the 1× RRB buffer is 8.3 ± 0.2 @ 25°C.

2.   Electrophoresis

a.   Fast electrophoresis: 1.5% agarose is recommended. DNA fragments (100-5000 bp) can be separated effectively in 10 minutes under the voltage of 300-350 V (15 V/cm).

b.   General electrophoresis: 1× RRB buffer can replace 1× TBE buffer for agarose gel electrophoresis of small-sized DNA.

Components
Components HY-K1017-10 pouches HY-K1017-50 pouches
Rapid Running Buffer Powder (1 L of 1×) 10 pouches 5 × 10 pouches
Documentation

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Product Name:
Rapid Running Buffer Powder (1 L of 1×)
Cat. No.:
HY-K1017
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