1. MCE Kits
  2. Cell Biology
  3. Cell Analysis
  4. Cell Apoptosis and Necrosis
  5. VF 647A-Annexin V/PI Apoptosis Detection Kit

VF 647A-Annexin V/PI Apoptosis Detection Kit 

Cat. No.: HY-K1093
Manual SDS

MCE VF 647A-Annexin V/PI Apoptosis Detection Kit can be utilized to detect the level of cell apoptosis.

VF 647A-Annexin V/PI Apoptosis Detection Kit
Size Price Stock Quantity
Free Sample (10 T)   Apply Now  
50 T USD 159 In-stock
100 T USD 272 In-stock

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

Apoptosis is a form of programmed cell death. Based on the cellular changes during programmed cell death, apoptosis is often classified into early, middle, and later stages. The later stages of apoptosis are characterized by changes in nuclear morphology, chromatin condensation, nuclear envelope degradation, and DNA fragmentation.

Annexin V is a 35-36 kDa Ca2+ - dependent phospholipid-binding protein that has a high affinity for PS. In normal live cells, Phosphatidylserine (PS) is located on the inner side of the cell membrane. Upon initiation of apoptosis, PS translocates from the inner to the outer leaflet of the membrane and binds with Annexin V. Therefore, Annexin V serves as one of the sensitive indicator of early-stage apoptosis. Once labeled with a fluorescent probe, Annexin V can be used to detect the early-stage apoptosis using fluorescence microscopy or flow cytometry. Propidium Iodide (PI) is a cell-membrane impermeable dye that can stain late-stage apoptosis cells and necrosis cells with red fluorescence.

 

MCE VF 647A-Annexin V/PI Apoptosis Detection Kit can be utilized to detect the level of cell apoptosis. After co-staining with Annexin V (labeled with a far-red fluorescent probe (VF 647A)) and PI, normal cells are essentially non-fluorescent (Annexin V-/PI-), early-stage apoptosis cells show far-red fluorescence (Annexin V+/PI-), late-stage apoptosis cells and necrosis cells show red fluorescence (Annexin V+/PI+).

Storage

4℃, 2 year

Keep away from light, do not freeze.

Components
Components HY-K1093-50 T HY-K1093-100 T
Annexin V Binding Buffer 50 mL 50 mL × 2
VF 647A-Annexin V 250 μL 500 μL
PI Staining Solution 500 μL 1 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
VF 647A-Annexin V/PI Apoptosis Detection Kit
Cat. No.:
HY-K1093
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